CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022676
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Acyl-coenzyme A thioesterase 2, mitochondrial 
Protein Synonyms/Alias
 Acyl-CoA thioesterase 2; Acyl coenzyme A thioester hydrolase; MTE-I; Very-long-chain acyl-CoA thioesterase 
Gene Name
 Acot2 
Gene Synonyms/Alias
 Mte1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
83RSALRDEKGALFRAHacetylation[1, 2, 3, 4, 5, 6, 7]
344LQSPLVDKKSFIPVEacetylation[6]
447QLQTFFHKQLGSKS*acetylation[7, 8]
447QLQTFFHKQLGSKS*succinylation[8]
Reference
 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [7] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [8] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337
Functional Description
 Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Most active on substrates with chain lengths ranging from C14-C20 (By similarity). 
Sequence Annotation
 ACT_SITE 273 273 Charge relay system (By similarity).
 ACT_SITE 365 365 Charge relay system (By similarity).
 ACT_SITE 399 399 Charge relay system (By similarity).  
Keyword
 Complete proteome; Hydrolase; Mitochondrion; Reference proteome; Serine esterase; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 453 AA 
Protein Sequence
MVASSFAVLR ASRLCQQDWK SWARLFVPPP LSTGGRTTWA RTNATLSVEP EGRSCWDEPL 60
SIAVRGLAPE QPVTLRSALR DEKGALFRAH ARYRADAGGE LNLARAPALG GSFSGLEPMG 120
LLWAMEPERP LWRLIKRDVQ TPFLVELEVL DGHEPDGGQR LAQAVHERHF LAPGVRRVPV 180
REGRVRATLF LPPEPGPFPG IIDLFGVGGG LLEYRASLLA GKGFAVMALA YYNYDDLPKS 240
IETMHMEYFE EAVNYLRSHP EVKGPGIGLL GISKGGELGL AMASFLKGIT AAVVINGSVA 300
AVGNTISYKD ETIPPVSLLR NQVKMTKDGL LDVVEALQSP LVDKKSFIPV ERSDTTFLFL 360
VGQDDHNWKS EFYADEISKR LQAHGKEKPQ IICYPAAGHY IEPPYFPLCS AGMHLLVGAN 420
ITFGGEPRAH AVAQVDAWQQ LQTFFHKQLG SKS 453 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0047617; F:acyl-CoA hydrolase activity; TAS:MGI.
 GO:0004091; F:carboxylesterase activity; IEA:UniProtKB-KW.
 GO:0016290; F:palmitoyl-CoA hydrolase activity; IEA:EC.
 GO:0006637; P:acyl-CoA metabolic process; TAS:MGI. 
Interpro
 IPR016662; Acyl-CoA_thioEstase_long-chain.
 IPR014940; BAAT_C.
 IPR006862; Thio_Ohase/aa_AcTrfase. 
Pfam
 PF08840; BAAT_C
 PF04775; Bile_Hydr_Trans 
SMART
  
PROSITE
  
PRINTS