CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030494
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vacuolar protein sorting 33B (Yeast), isoform CRA_b 
Protein Synonyms/Alias
 Vacuolar protein sorting-associated protein 33B; cDNA FLJ90382 fis, clone NT2RP2005069, highly similar to Vacuolar protein sorting 33B 
Gene Name
 VPS33B 
Gene Synonyms/Alias
 hCG_32582 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
12ASLVNADKLAGRTRKubiquitination[1]
110YGIGRCAKMAYELWRubiquitination[2]
128EEEDGETKGRRPEIGubiquitination[1, 3]
167VDDTFRIKCGSVDFGubiquitination[2]
213VFGFLSQKARNLQAQubiquitination[2, 4]
367TLTAVESKVSKLVTDubiquitination[2, 3]
370AVESKVSKLVTDKAAubiquitination[2]
375VSKLVTDKAAGKITDubiquitination[2]
389DAFSSLAKRSNFRAIubiquitination[2, 3]
413VDGEYDLKVPRDMAYubiquitination[2, 3]
525MEAMSEVKA******ubiquitination[2]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 526 AA 
Protein Sequence
MRYIASLVNA DKLAGRTRKY KVIFSPQKFY ACEMVLEEEG IYGDVSCDEW AFSLLPLDVD 60
LLSMELPEFF RDYFLEGDQR WINTVAQALH LLSTLYGPFP NCYGIGRCAK MAYELWRNLE 120
EEEDGETKGR RPEIGHIFLL DRDVDFVTAL CSQVVYEGLV DDTFRIKCGS VDFGPEVTSS 180
DKSLKVLLNA EDKVFNEIRN EHFSNVFGFL SQKARNLQAQ YDRRRGMDIK QMKNFVSQEL 240
KGLKQEHRLL SLHIGACESI MKKKTKQDFQ ELIKTEHALL EGFNIRESTS YIEEHIDRQV 300
SPIESLRLMC LLSITENGLI PKDYRSLKTQ YLQSYGPEHL LTFSNLRRAG LLTEQAPGDT 360
LTAVESKVSK LVTDKAAGKI TDAFSSLAKR SNFRAISKKL NLIPRVDGEY DLKVPRDMAY 420
VFGGAYVPLS CRIIEQVLER RSWQGLDEVV RLLNCSDFAF TDMTKEDKAS SESLRLILVV 480
FLGGCTFSEI SALRFLGREK GYRFIFLTTA VTNSARLMEA MSEVKA 526 
Gene Ontology
 GO:0006904; P:vesicle docking involved in exocytosis; IEA:InterPro. 
Interpro
 IPR027482; Sec-1-like_dom2.
 IPR001619; Sec1-like.
 IPR027121; VPS33. 
Pfam
 PF00995; Sec1 
SMART
  
PROSITE
  
PRINTS