CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013516
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Putative heat shock protein HSP 90-beta 2 
Protein Synonyms/Alias
 Heat shock protein 90-beta b; Heat shock protein 90Bb 
Gene Name
 HSP90AB2P 
Gene Synonyms/Alias
 HSP90BB 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
69PSKLDSGKELKIDIIubiquitination[1]
141PITLYLEKEREKEISacetylation[2]
141PITLYLEKEREKEISubiquitination[1, 3, 4, 5, 6, 7]
197KTKKIKEKYIDQEELubiquitination[1]
206IDQEELNKTKPIWTRubiquitination[1]
208QEELNKTKPIWTRNTubiquitination[1, 5]
304QLKEFDGKSLVSVTKubiquitination[1]
332RMEERKAKFENLCKFubiquitination[1, 5]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Putative molecular chaperone that may promote the maturation, structural maintenance and proper regulation of specific target proteins (By similarity). 
Sequence Annotation
 BINDING 46 46 ATP (By similarity).
 BINDING 88 88 ATP (By similarity).
 BINDING 107 107 ATP (By similarity).
 MOD_RES 177 177 Phosphoserine.  
Keyword
 ATP-binding; Chaperone; Coiled coil; Complete proteome; Cytoplasm; Nucleotide-binding; Phosphoprotein; Reference proteome; Stress response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 381 AA 
Protein Sequence
MPEEVHLGEK EVETFAFQAE IAQLMSLIIN TFYSNKEIFL WELISNASDA LDKIRYESLT 60
DPSKLDSGKE LKIDIIPNTQ EHTLTLVDTG IGMTKADLIN NLGTIAKFQD QTEYLEEMQV 120
KEVVEKHSQF LGYPITLYLE KEREKEISDG KAEEEKGEKE EENKDDEEKP KIEDVGSDEE 180
DDSGKDKKKK TKKIKEKYID QEELNKTKPI WTRNTEDITQ EEYGEFYKSL TNDWKDHLAV 240
RYFSVEEYVS RMKEIQKSIY YITGESKEQV ANSAFVEQVW KRDSRVVYMT EPIDGYQLKE 300
FDGKSLVSVT KEGLELPEDG EEKKRMEERK AKFENLCKFM KETLDKKVEM VTVSNRLVSS 360
SCCIVTSTYS WTANMEQIMK A 381 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006457; P:protein folding; IEA:InterPro.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW. 
Interpro
 IPR003594; HATPase_ATP-bd.
 IPR001404; Hsp90.
 IPR020575; Hsp90_N.
 IPR020568; Ribosomal_S5_D2-typ_fold. 
Pfam
 PF00183; HSP90 
SMART
 SM00387; HATPase_c 
PROSITE
 PS00298; HSP90 
PRINTS
 PR00775; HEATSHOCK90.