UniProt Accession

 UVRB_ECOLI; P0A8F8; P07025 

Genbank Protein ID

 X03678; X03722; U00096; AP009048 

Genbank Nucleotide ID

 CAA27314.1; CAA27357.1; AAC73866.1; BAA35437.2 

Protein Name

 UvrABC system protein B 

Protein Synonyms/Alias

 Protein UvrB; Excinuclease ABC subunit B 

Gene Name

 uvrB 

Gene Synonyms/Alias

 b0779; JW0762 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
12	FKLNSAFKPSGDQPE	acetylation	[1]
264	VQAMEEIKEELAARR	acetylation	[1]
378	ALDNRPLKFEEFEAL	acetylation	[1]
456	VLVTTLTKRMAEDLT	acetylation	[1]
556	KAILYGDKITPSMAK	acetylation	[1]
592	TPQGLNKKVVDILAL	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage. 

Sequence Annotation

 DOMAIN 26 415 Helicase ATP-binding.
 DOMAIN 431 597 Helicase C-terminal.
 DOMAIN 633 668 UVR.
 NP_BIND 39 46 ATP (Potential).
 MOTIF 92 115 Beta-hairpin.  

Keyword

 3D-structure; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; Reference proteome; SOS response. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 673 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0003677; F:DNA binding; IEA:HAMAP.
 GO:0009381; F:excinuclease ABC activity; IEA:HAMAP.
 GO:0004386; F:helicase activity; IEA:HAMAP.
 GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
 GO:0006289; P:nucleotide-excision repair; IEA:HAMAP.
 GO:0009432; P:SOS response; IEA:HAMAP. 

Interpro

 IPR006935; Helicase/UvrB_dom.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR001943; UVR_dom.
 IPR004807; UvrB.
 IPR024759; UvrB_YAD/RRR_dom. 

Pfam

 PF00271; Helicase_C
 PF04851; ResIII
 PF02151; UVR
 PF12344; UvrB 

SMART

 SM00487; DEXDc
 SM00490; HELICc 

PROSITE

 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS50151; UVR 

PRINTS