UniProt Accession

 F162A_HUMAN; Q96A26; Q9NRN6; Q9UJX8 

Genbank Protein ID

 AF191020; AF201944; AF107495; BC010896; BC015060 

Genbank Nucleotide ID

 AAF09484.1; AAF86880.1; AAG39275.1; AAH10896.1; AAH15060.1 

Protein Name

 Protein FAM162A 

Protein Synonyms/Alias

 E2-induced gene 5 protein; Growth and transformation-dependent protein; HGTD-P 

Gene Name

 FAM162A 

Gene Synonyms/Alias

 C3orf28; E2IG5; DC16; FWP001 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
79	IWSGRFKKEDEIPET	ubiquitination	[1]
96	LEMLDAAKNKMRVKI	ubiquitination	[1]
98	MLDAAKNKMRVKISY	ubiquitination	[1]
139	LTSLNLEKKARLKEE	ubiquitination	[1, 2]
140	TSLNLEKKARLKEEA	ubiquitination	[3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Proposed to be involved in regulation of apoptosis; the exact mechanism may differ between cell types/tissues. May be involved in hypoxia-induced cell death of transformed cells implicating cytochrome C release and caspase activation (such as CASP9) and inducing mitochondrial permeability transition. May be involved in hypoxia-induced cell death of neuronal cells probably by promoting release of AIFM1 from mitochondria to cytoplasm and its translocation to the nucleus; however, the involvement of caspases has been reported conflictingly. 

Sequence Annotation

 REGION 76 102 Required for proapoptotic activity.  

Keyword

 Apoptosis; Complete proteome; Membrane; Mitochondrion; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 154 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; IDA:UniProtKB.
 GO:0071456; P:cellular response to hypoxia; IDA:UniProtKB.
 GO:0051402; P:neuron apoptotic process; IEA:Compara.
 GO:0043065; P:positive regulation of apoptotic process; IDA:UniProtKB.
 GO:0090200; P:positive regulation of release of cytochrome c from mitochondria; IDA:UniProtKB.
 GO:0006927; P:transformed cell apoptotic process; IDA:UniProtKB. 

Interpro

 IPR009432; DUF1075. 

Pfam

 PF06388; DUF1075 

SMART

  

PROSITE

  

PRINTS