CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030852
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Methyl-CpG-binding domain protein 1 
Protein Synonyms/Alias
 cDNA FLJ55972, highly similar to Methyl-CpG-binding domain protein 1 
Gene Name
 MBD1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
328RKGGCDSKMAARRRPubiquitination[1]
388TNRRQNRKCGACAACubiquitination[2]
473HHLGPTLKPTLATRTacetylation[3, 4]
473HHLGPTLKPTLATRTubiquitination[1, 4, 5, 6]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 656 AA 
Protein Sequence
MGGFRLRTGR NAALNSDRLL LLPVASMAED WLDCPALGPG WKRREVFRKS GATCGRSDTY 60
YQSPTGDRIR SKVELTRYLG PACDLTLFDF KQGILCYPAP KAHPVAVASK KRKKPSRPAK 120
TRKRQVGPQS GEVRKEAPRD ETKADTDTAP ASFPAPGCCE NCGISFSGDG TQRQRLKTLC 180
KDCRAQRIAF NREQRMFKRV GCGECAACQV TEDCGACSTC LLQLPHDVAS GLFCKCERRR 240
CLRIVERSRG CGVCRGCQTQ EDCGHCPICL RPPRPGLRRQ WKCVQRRCLR HLAHRLRRRH 300
QRCQRRTPLA VAPPTGKHAR RKGGCDSKMA ARRRPGAQPL PPPPPSQSPE PTEPHPRALA 360
PSPPAEFIYY CVDEDELQPY TNRRQNRKCG ACAACLRRMD CGRCDFCCDK PKFGGSNQKR 420
QKCRWRQCLQ FAMKRLLPSV WSESEDGAGS PPPYRRRKRP SSARRHHLGP TLKPTLATRT 480
AQPDHTQAPT KQEAGGGFVL PPPGTDLVFL REGASSPVQV PGPVAASTEA LLQEAQCSGL 540
SWVVALPQVK QEKADTQDEW TPGTAVLTSP VLVPGCPSKA VDPGLPSVKQ EPPDPEEDKE 600
ENKDDSASKL APEEEAGGAG TPVITEIFSL GGTRFRDTAV WLPRSKDLKK PGARKQ 656 
Gene Ontology
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR016177; DNA-bd_integrase-typ.
 IPR001739; Methyl_CpG_DNA-bd.
 IPR002857; Znf_CXXC. 
Pfam
 PF01429; MBD
 PF02008; zf-CXXC 
SMART
 SM00391; MBD 
PROSITE
 PS50982; MBD
 PS51058; ZF_CXXC 
PRINTS