UniProt Accession

 TKT1_YEAST; P23254; D6W478 

Genbank Protein ID

 M63302; X73224; Z49219; Z71255; U51033; BK006949 

Genbank Nucleotide ID

 AAA35168.1; CAA51693.1; CAA89191.1; CAA94982.1; AAB68125.1; DAA11494.1 

Protein Name

 Transketolase 1 

Protein Synonyms/Alias

 TK 1 

Gene Name

 TKL1 

Gene Synonyms/Alias

 YPR074C; YP9499.29C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
268	SVHGAPLKADDVKQL	acetylation	[1]
273	PLKADDVKQLKSKFG	acetylation	[1]
278	DVKQLKSKFGFNPDK	acetylation	[1]
285	KFGFNPDKSFVVPQE	ubiquitination	[2]
299	EVYDHYQKTILKPGV	acetylation	[1]
303	HYQKTILKPGVEANN	acetylation	[1]
303	HYQKTILKPGVEANN	ubiquitination	[2]
311	PGVEANNKWNKLFSE	acetylation	[1]
314	EANNKWNKLFSEYQK	acetylation	[1]
321	KLFSEYQKKFPELGA	acetylation	[1]
322	LFSEYQKKFPELGAE	acetylation	[1]
352	KLPTYTAKDSAVATR	acetylation	[1]
352	KLPTYTAKDSAVATR	ubiquitination	[2]
360	DSAVATRKLSETVLE	ubiquitination	[2]
392	PSNLTRWKEALDFQP	ubiquitination	[2]
518	YKNSLESKHTPSIIA	acetylation	[1]
641	DRFGASGKAPEVFKF	acetylation	[1]
641	DRFGASGKAPEVFKF	ubiquitination	[2]
647	GKAPEVFKFFGFTPE	acetylation	[1]
647	GKAPEVFKFFGFTPE	ubiquitination	[3]
662	GVAERAQKTIAFYKG	acetylation	[1]
668	QKTIAFYKGDKLISP	acetylation	[1]
668	QKTIAFYKGDKLISP	ubiquitination	[2]
671	IAFYKGDKLISPLKK	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate. 

Sequence Annotation

 NP_BIND 116 118 Thiamine pyrophosphate.
 ACT_SITE 418 418 Proton donor (Probable).
 METAL 157 157 Magnesium.
 METAL 187 187 Magnesium.
 METAL 189 189 Magnesium; via carbonyl oxygen.
 BINDING 30 30 Substrate.
 BINDING 69 69 Thiamine pyrophosphate.
 BINDING 158 158 Thiamine pyrophosphate; via amide
 BINDING 187 187 Thiamine pyrophosphate.
 BINDING 263 263 Substrate.
 BINDING 263 263 Thiamine pyrophosphate.
 BINDING 359 359 Substrate.
 BINDING 386 386 Substrate.
 BINDING 418 418 Thiamine pyrophosphate.
 BINDING 445 445 Thiamine pyrophosphate.
 BINDING 469 469 Substrate.
 BINDING 477 477 Substrate.
 BINDING 528 528 Substrate.
 MOD_RES 286 286 Phosphoserine.
 MOD_RES 335 335 Phosphoserine.
 MOD_RES 402 402 Phosphoserine.
 MOD_RES 492 492 Phosphoserine.  

Keyword

 3D-structure; Calcium; Complete proteome; Direct protein sequencing; Magnesium; Metal-binding; Phosphoprotein; Reference proteome; Thiamine pyrophosphate; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 680 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; ISS:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004802; F:transketolase activity; IMP:SGD.
 GO:0006098; P:pentose-phosphate shunt; IMP:SGD. 

Interpro

 IPR009014; Transketo_C/Pyr-ferredox_oxred.
 IPR015941; Transketolase-like_C.
 IPR005475; Transketolase-like_Pyr-bd.
 IPR005478; Transketolase_bac-like.
 IPR020826; Transketolase_BS.
 IPR005476; Transketolase_C.
 IPR005474; Transketolase_N. 

Pfam

 PF02779; Transket_pyr
 PF02780; Transketolase_C
 PF00456; Transketolase_N 

SMART

 SM00861; Transket_pyr 

PROSITE

 PS00801; TRANSKETOLASE_1
 PS00802; TRANSKETOLASE_2 

PRINTS