CPLM-012483

Genbank Nucleotide ID

Eukaryotic translation initiation factor 4H

Protein Synonyms/Alias

eIF-4H; Williams-Beuren syndrome chromosomal region 1 protein

KIAA0038; WBSCR1; WSCR1

Homo sapiens (Human)

Position	Peptide	Type	References
80	VRDKDTDKFKGFCYV	acetylation	[1]
80	VRDKDTDKFKGFCYV	ubiquitination	[2]
120	VDIAEGRKQDKGGFG	ubiquitination	[3]
123	AEGRKQDKGGFGFRK	ubiquitination	[3, 4]
214	QRPRLQLKPRTVATP	acetylation	[5]
214	QRPRLQLKPRTVATP	ubiquitination	[3]
245	PREEVVQKEQE****	acetylation	[1, 6, 7]
245	PREEVVQKEQE****	ubiquitination	[1, 2, 4]

[1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[5] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
[6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]

Functional Description

Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.

DOMAIN 42 118 RRM.
REGION 137 157 HHV-1 Vhs binding site.
MOD_RES 2 2 N-acetylalanine.
MOD_RES 21 21 Phosphoserine.
MOD_RES 24 24 Phosphoserine.
MOD_RES 32 32 Phosphoserine.

Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Host-virus interaction; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding; Williams-Beuren syndrome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0016281; C:eukaryotic translation initiation factor 4F complex; TAS:ProtInc.
GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
GO:0000166; F:nucleotide binding; IEA:InterPro.
GO:0003743; F:translation initiation factor activity; TAS:ProtInc.
GO:0048589; P:developmental growth; IEA:Compara.
GO:0006446; P:regulation of translational initiation; TAS:ProtInc.
GO:0019953; P:sexual reproduction; IEA:Compara.
GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW.

IPR012677; Nucleotide-bd_a/b_plait.
IPR000504; RRM_dom.