CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021718
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NADPH oxidase 4 
Protein Synonyms/Alias
 Kidney oxidase-1; KOX-1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase; Superoxide-generating NADPH oxidase 4 
Gene Name
 Nox4 
Gene Synonyms/Alias
 Renox 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
328VMELRMIKENFKARPubiquitination[1]
566FIGRPRWKLLFDEIAubiquitination[1]
608RNNSYGTKFEYNKESubiquitination[1]
613GTKFEYNKESFS***ubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. 
Sequence Annotation
 DOMAIN 58 303 Ferric oxidoreductase.
 DOMAIN 304 419 FAD-binding FR-type.
 REGION 248 575 Mediates interaction with TLR4 (By
 CARBOHYD 133 133 N-linked (GlcNAc...) (Potential).
 CARBOHYD 230 230 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Cell junction; Cell membrane; Complete proteome; Disulfide bond; Endoplasmic reticulum; Glycoprotein; Membrane; NADP; Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 578 AA 
Protein Sequence
MAVSWRSWLA NEGVKHLCLL IWLSLNVLLF WKTFLLYNQG PEYYYIHQML GLGLCLSRAS 60
ASVLNLNCSL ILLPMCRTVL AYLRGSQKVP SRRTRRLLDK SKTLHITCGV TICIFSGVHV 120
AAHLVNALNF SVNYSEDFLE LNAARYQNED PRKLLFTTIP GLTGVCMVVV LFLMVTASTY 180
AIRVSNYDIF WYTHNLFFVF YMLLLLHVSG GLLKYQTNVD THPPGCISLN QTSSQNMSIP 240
DYVSEHFHGS LPRGFSKLED RYQKTLVKIC LEEPKFQAHF PQTWIWISGP LCLYCAERLY 300
RCIRSNKPVT IISVINHPSD VMELRMIKEN FKARPGQYII LHCPSVSALE NHPFTLTMLC 360
QKRWQPWHSR VWIYSFFVCS DAACHEDTSK MNHAFLLCPT ETKATFGVHF KVVGDWTERF 420
RDLLLPPSSQ DSEILPFIHS RNYPKLYIDG PFGSPFEESL NYEVSLCVAG GIGVTPFASI 480
LNTLLDDWKP YKLRRLYFIW VCRDIQSFQW FADLLCVLHN KFWQENRPDF VNIQLYLSQT 540
DGIQKIIGEK YHTLNSRLFI GRPRWKLLFD EIAKCNRGKT VGVFCCGPSS ISKTLHSLSN 600
RNNSYGTKFE YNKESFS 617 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IEA:Compara.
 GO:0071944; C:cell periphery; IDA:UniProtKB.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005925; C:focal adhesion; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0043020; C:NADPH oxidase complex; IEA:Compara.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
 GO:0072341; F:modified amino acid binding; IEA:Compara.
 GO:0016174; F:NAD(P)H oxidase activity; IEA:Compara.
 GO:0016175; F:superoxide-generating NADPH oxidase activity; IMP:UniProtKB.
 GO:0045453; P:bone resorption; IMP:MGI.
 GO:0035051; P:cardiocyte differentiation; IMP:MGI.
 GO:0007569; P:cell aging; IMP:UniProtKB.
 GO:0000902; P:cell morphogenesis; IMP:UniProtKB.
 GO:0071320; P:cellular response to cAMP; IEA:Compara.
 GO:0071480; P:cellular response to gamma radiation; IEA:Compara.
 GO:0071333; P:cellular response to glucose stimulus; IEP:UniProtKB.
 GO:0071560; P:cellular response to transforming growth factor beta stimulus; IEA:Compara.
 GO:0050667; P:homocysteine metabolic process; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; IMP:UniProtKB.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:2000573; P:positive regulation of DNA biosynthetic process; IMP:UniProtKB.
 GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IMP:UniProtKB.
 GO:0043406; P:positive regulation of MAP kinase activity; IMP:UniProtKB.
 GO:0051897; P:positive regulation of protein kinase B signaling cascade; IMP:UniProtKB.
 GO:2000379; P:positive regulation of reactive oxygen species metabolic process; IEA:Compara.
 GO:0014911; P:positive regulation of smooth muscle cell migration; IEA:Compara.
 GO:0051496; P:positive regulation of stress fiber assembly; IEA:Compara.
 GO:0001666; P:response to hypoxia; IEA:Compara.
 GO:0042554; P:superoxide anion generation; IDA:UniProtKB. 
Interpro
 IPR000778; Cyt_b245_heavy_chain.
 IPR013112; FAD-bd_8.
 IPR017927; Fd_Rdtase_FAD-bd.
 IPR013130; Fe3_Rdtase_TM_dom.
 IPR013121; Fe_red_NAD-bd_6.
 IPR017938; Riboflavin_synthase-like_b-brl. 
Pfam
 PF08022; FAD_binding_8
 PF01794; Ferric_reduct
 PF08030; NAD_binding_6 
SMART
  
PROSITE
 PS51384; FAD_FR 
PRINTS
 PR00466; GP91PHOX.