CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013976
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable inactive glycosyltransferase 25 family member 3 
Protein Synonyms/Alias
 Cerebral endothelial cell adhesion molecule 
Gene Name
 CERCAM 
Gene Synonyms/Alias
 CEECAM1; GLT25D3; KIAA1502 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
126HQFLMELKQEALTFAubiquitination[1]
200TAEYFPTKNRQRRGCubiquitination[1, 2, 3, 4]
275GYMNVPVKSHQGLEDubiquitination[2]
438MEDVEAEKLSWDLIYubiquitination[2]
570ISWSGSQKTLRSPRLubiquitination[5, 6]
Reference
 [1] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Has apparently no beta-galactosyltransferase activity. 
Sequence Annotation
 MOTIF 592 595 Prevents secretion from ER (Potential).
 CARBOHYD 75 75 N-linked (GlcNAc...) (Potential).
 CARBOHYD 153 153 N-linked (GlcNAc...) (Potential).
 CARBOHYD 237 237 N-linked (GlcNAc...) (Potential).
 CARBOHYD 360 360 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Cell adhesion; Complete proteome; Endoplasmic reticulum; Glycoprotein; Reference proteome; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 595 AA 
Protein Sequence
MRAARAAPLL QLLLLLGPWL EAAGVAESPL PAVVLAILAR NAEHSLPHYL GALERLDYPR 60
ARMALWCATD HNVDNTTEML QEWLAAVGDD YAAVVWRPEG EPRFYPDEEG PKHWTKERHQ 120
FLMELKQEAL TFARNWGADY ILFADTDNIL TNNQTLRLLM GQGLPVVAPM LDSQTYYSNF 180
WCGITPQGYY RRTAEYFPTK NRQRRGCFRV PMVHSTFLAS LRAEGADQLA FYPPHPNYTW 240
PFDDIIVFAY ACQAAGVSVH VCNEHRYGYM NVPVKSHQGL EDERVNFIHL ILEALVDGPR 300
MQASAHVTRP SKRPSKIGFD EVFVISLARR PDRRERMLAS LWEMEISGRV VDAVDGWMLN 360
SSAIRNLGVD LLPGYQDPYS GRTLTKGEVG CFLSHYSIWE EVVARGLARV LVFEDDVRFE 420
SNFRGRLERL MEDVEAEKLS WDLIYLGRKQ VNPEKETAVE GLPGLVVAGY SYWTLAYALR 480
LAGARKLLAS QPLRRMLPVD EFLPIMFDQH PNEQYKAHFW PRDLVAFSAQ PLLAAPTHYA 540
GDAEWLSDTE TSSPWDDDSG RLISWSGSQK TLRSPRLDLT GSSGHSLQPQ PRDEL 595 
Gene Ontology
 GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; NAS:ProtInc.
 GO:0006928; P:cellular component movement; TAS:ProtInc.
 GO:0007159; P:leukocyte cell-cell adhesion; TAS:ProtInc.
 GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:InterPro. 
Interpro
 IPR002654; Glyco_trans_25. 
Pfam
 PF01755; Glyco_transf_25 
SMART
  
PROSITE
 PS00014; ER_TARGET 
PRINTS