UniProt Accession

 M3K11_HUMAN; Q16584; Q53H00; Q59F06; Q6P2G4 

Genbank Protein ID

 U07747; L32976; AB209655; AK222781; BC011263; BC064543 

Genbank Nucleotide ID

 AAA19647.1; AAA59859.1; BAD92892.1; BAD96501.1; AAH11263.1; AAH64543.1 

Protein Name

 Mitogen-activated protein kinase kinase kinase 11 

Protein Synonyms/Alias

 Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase 

Gene Name

 MAP3K11 

Gene Synonyms/Alias

 MLK3; PTK1; SPRK 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
264	DMEHKTLKITDFGLA	ubiquitination	[1]
499	ISMPLDFKHRITVQA	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle. 

Sequence Annotation

 DOMAIN 41 105 SH3.
 DOMAIN 117 379 Protein kinase.
 NP_BIND 123 131 ATP (By similarity).
 REGION 403 424 Leucine-zipper 1.
 REGION 438 459 Leucine-zipper 2.
 ACT_SITE 241 241 Proton acceptor (By similarity).
 BINDING 144 144 ATP.
 MOD_RES 11 11 Phosphoserine.
 MOD_RES 277 277 Phosphothreonine; by autocatalysis.
 MOD_RES 281 281 Phosphoserine; by autocatalysis and
 MOD_RES 394 394 Phosphoserine.
 MOD_RES 507 507 Phosphoserine.
 MOD_RES 524 524 Phosphoserine.
 MOD_RES 548 548 Phosphoserine.
 MOD_RES 555 555 Phosphoserine.
 MOD_RES 556 556 Phosphoserine.
 MOD_RES 654 654 Phosphoserine.
 MOD_RES 693 693 Phosphoserine.
 MOD_RES 705 705 Phosphoserine.
 MOD_RES 708 708 Phosphothreonine.
 MOD_RES 724 724 Phosphoserine.
 MOD_RES 727 727 Phosphoserine.
 MOD_RES 740 740 Phosphoserine.
 MOD_RES 748 748 Phosphoserine.
 MOD_RES 758 758 Phosphoserine.
 MOD_RES 770 770 Phosphoserine.
 MOD_RES 789 789 Phosphoserine.
 MOD_RES 793 793 Phosphoserine.
 MOD_RES 815 815 Phosphoserine.  

Keyword

 ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Serine/threonine-protein kinase; SH3 domain; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 847 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005874; C:microtubule; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004706; F:JUN kinase kinase kinase activity; ISS:UniProtKB.
 GO:0004713; F:protein tyrosine kinase activity; IEA:InterPro.
 GO:0007257; P:activation of JUN kinase activity; IMP:UniProtKB.
 GO:0008219; P:cell death; IEA:Compara.
 GO:0008283; P:cell proliferation; IMP:UniProtKB.
 GO:0000080; P:G1 phase of mitotic cell cycle; IMP:UniProtKB.
 GO:0007017; P:microtubule-based process; IMP:UniProtKB.
 GO:0046330; P:positive regulation of JNK cascade; IMP:UniProtKB.
 GO:0043525; P:positive regulation of neuron apoptotic process; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; IDA:UniProtKB. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR016231; MAPKKK9/10/11.
 IPR015785; MAPKKK9/10/11-like.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR008271; Ser/Thr_kinase_AS.
 IPR011511; SH3_2.
 IPR001452; SH3_domain.
 IPR020635; Tyr_kinase_cat_dom. 

Pfam

 PF07714; Pkinase_Tyr
 PF07653; SH3_2 

SMART

 SM00326; SH3
 SM00219; TyrKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS50002; SH3 

PRINTS

 PR00452; SH3DOMAIN.
 PR00109; TYRKINASE.