CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018743
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Polyphosphoinositide phosphatase 
Protein Synonyms/Alias
 Phosphatidylinositol 3,5-bisphosphate 5-phosphatase; SAC domain-containing protein 3 
Gene Name
 FIG4 
Gene Synonyms/Alias
 KIAA0274; SAC3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
35GSNNAETKYRVLKIDubiquitination[1]
81NRTKMGQKGSSGLFRubiquitination[1]
190RMPLEMLKSEMTQNRubiquitination[2]
379IIILNLVKEREKRKHubiquitination[1, 3]
425MAKYTKSKLCNVLDRubiquitination[1]
443IAESVVKKTGFFVNRubiquitination[1]
658ICAVNLKKLIVKKFHubiquitination[1]
704SARDFMPKTVGIDPSubiquitination[1]
833QSQHKQDKNSQQPCSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
 The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). In vitro, hydrolyzes all three D5-phosphorylated polyphosphoinositide substrates in the order PtdIns(4,5)P2 > PtdIns(3,5)P2 > PtdIns(3,4,5)P3. Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes. 
Sequence Annotation
 DOMAIN 154 547 SAC.  
Keyword
 Amyotrophic lateral sclerosis; Charcot-Marie-Tooth disease; Complete proteome; Disease mutation; Endosome; Hydrolase; Membrane; Neurodegeneration; Neuropathy; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 907 AA 
Protein Sequence
MPTAAAPIIS SVQKLVLYET RARYFLVGSN NAETKYRVLK IDRTEPKDLV IIDDRHVYTQ 60
QEVRELLGRL DLGNRTKMGQ KGSSGLFRAV SAFGVVGFVR FLEGYYIVLI TKRRKMADIG 120
GHAIYKVEDT NMIYIPNDSV RVTHPDEARY LRIFQNVDLS SNFYFSYSYD LSHSLQYNLT 180
VLRMPLEMLK SEMTQNRQES FDIFEDEGLI TQGGSGVFGI CSEPYMKYVW NGELLDIIKS 240
TVHRDWLLYI IHGFCGQSKL LIYGRPVYVT LIARRSSKFA GTRFLKRGAN CEGDVANEVE 300
TEQILCDASV MSFTAGSYSS YVQVRGSVPL YWSQDISTMM PKPPITLDQA DPFAHVAALH 360
FDQMFQRFGS PIIILNLVKE REKRKHERIL SEELVAAVTY LNQFLPPEHT IVYIPWDMAK 420
YTKSKLCNVL DRLNVIAESV VKKTGFFVNR PDSYCSILRP DEKWNELGGC VIPTGRLQTG 480
ILRTNCVDCL DRTNTAQFMV GKCALAYQLY SLGLIDKPNL QFDTDAVRLF EELYEDHGDT 540
LSLQYGGSQL VHRVKTYRKI APWTQHSKDI MQTLSRYYSN AFSDADRQDS INLFLGVFHP 600
TEGKPHLWEL PTDFYLHHKN TMRLLPTRRS YTYWWTPEVI KHLPLPYDEV ICAVNLKKLI 660
VKKFHKYEEE IDIHNEFFRP YELSSFDDTF CLAMTSSARD FMPKTVGIDP SPFTVRKPDE 720
TGKSVLGNKS NREEAVLQRK TAASAPPPPS EEAVSSSSED DSGTDREEEG SVSQRSTPVK 780
MTDAGDSAKV TENVVQPMKE LYGINLSDGL SEEDFSIYSR FVQLGQSQHK QDKNSQQPCS 840
RCSDGVIKLT PISAFSQDNI YEVQPPRVDR KSTEIFQAHI QASQGIMQPL GKEDSSMYRE 900
YIRNRYL 907 
Gene Ontology
 GO:0031901; C:early endosome membrane; TAS:Reactome.
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0031902; C:late endosome membrane; TAS:Reactome.
 GO:0043813; F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity; TAS:Reactome.
 GO:0004438; F:phosphatidylinositol-3-phosphatase activity; IEA:Compara.
 GO:0043812; F:phosphatidylinositol-4-phosphate phosphatase activity; IEA:Compara.
 GO:0008219; P:cell death; IEA:UniProtKB-KW.
 GO:0007626; P:locomotory behavior; IEA:Compara.
 GO:0032288; P:myelin assembly; IEA:Compara.
 GO:0031642; P:negative regulation of myelination; IEA:Compara.
 GO:0048666; P:neuron development; IEA:Compara.
 GO:0043473; P:pigmentation; IEA:Compara.
 GO:0010976; P:positive regulation of neuron projection development; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0007033; P:vacuole organization; IEA:Compara. 
Interpro
 IPR002013; Syja_N. 
Pfam
 PF02383; Syja_N 
SMART
  
PROSITE
 PS50275; SAC 
PRINTS