CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018877
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 
Protein Synonyms/Alias
 60 kDa BRG-1/Brm-associated factor subunit B; BRG1-associated factor 60B; BAF60B 
Gene Name
 SMARCD2 
Gene Synonyms/Alias
 BAF60B; PRO2451 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
139RRGLKRRKMADKVLPubiquitination[1]
143KRRKMADKVLPQRIRubiquitination[1]
168DLLAFERKLDQTIARubiquitination[1]
185MEIQEAIKKPLTQKRacetylation[2]
185MEIQEAIKKPLTQKRubiquitination[1]
186EIQEAIKKPLTQKRKubiquitination[1]
206SNTFSPSKAEGDSAGubiquitination[1]
226GGTPAGDKVASWELRubiquitination[1]
237WELRVEGKLLDDPSKubiquitination[1, 3]
244KLLDDPSKQKRKFSSubiquitination[1]
246LDDPSKQKRKFSSFFubiquitination[1]
248DPSKQKRKFSSFFKSubiquitination[1]
262SLVIELDKELYGPDNubiquitination[1, 4]
289ETDGFQVKRPGDLNVubiquitination[1]
297RPGDLNVKCTLLLMLubiquitination[1]
312DHQPPQYKLDPRLARubiquitination[1]
376RFSEIPMKLAGLLQHubiquitination[1]
401SVDPNDQKKTACYDIubiquitination[1]
402VDPNDQKKTACYDIDubiquitination[1]
451IESINQLKTQRDFMLubiquitination[1, 4, 5, 6]
480RSQRRDLKIITDVIGubiquitination[1, 6]
514VGRHIFAKVQQRRQEubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). 
Sequence Annotation
 DOMAIN 307 382 SWIB.
 MOD_RES 217 217 Phosphothreonine.  
Keyword
 Alternative splicing; Chromatin regulator; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 531 AA 
Protein Sequence
MSGRGAGGFP LPPLSPGGGA VAAALGAPPP PAGPGMLPGP ALRGPGPAGG VGGPGAAAFR 60
PMGPAGPAAQ YQRPGMSPGN RMPMAGLQVG PPAGSPFGAA APLRPGMPPT MMDPFRKRLL 120
VPQAQPPMPA QRRGLKRRKM ADKVLPQRIR ELVPESQAYM DLLAFERKLD QTIARKRMEI 180
QEAIKKPLTQ KRKLRIYISN TFSPSKAEGD SAGTAGTPGG TPAGDKVASW ELRVEGKLLD 240
DPSKQKRKFS SFFKSLVIEL DKELYGPDNH LVEWHRMPTT QETDGFQVKR PGDLNVKCTL 300
LLMLDHQPPQ YKLDPRLARL LGVHTQTRAA IMQALWLYIK HNQLQDGHER EYINCNRYFR 360
QIFSCGRLRF SEIPMKLAGL LQHPDPIVIN HVISVDPNDQ KKTACYDIDV EVDDPLKAQM 420
SNFLASTTNQ QEIASLDVKI HETIESINQL KTQRDFMLSF STDPQDFIQE WLRSQRRDLK 480
IITDVIGNPE EERRAAFYHQ PWAQEAVGRH IFAKVQQRRQ ELEQVLGIRL T 531 
Gene Ontology
 GO:0016514; C:SWI/SNF complex; IDA:UniProtKB.
 GO:0003713; F:transcription coactivator activity; NAS:UniProtKB.
 GO:0006338; P:chromatin remodeling; IDA:BHF-UCL.
 GO:0006337; P:nucleosome disassembly; IDA:BHF-UCL.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; NAS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR003121; SWIB_MDM2_domain. 
Pfam
 PF02201; SWIB 
SMART
  
PROSITE
  
PRINTS