CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000881
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Histone-lysine N-methyltransferase, H3 lysine-9 specific 
Protein Synonyms/Alias
 Cryptic loci regulator 4; Histone H3-K9 methyltransferase; H3-K9-HMTase; Lysine N-methyltransferase 1 
Gene Name
 clr4 
Gene Synonyms/Alias
 kmt1; SPBC428.08c 
Created Date
 July 27, 2013 
Organism
 Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) 
NCBI Taxa ID
 284812 
Lysine Modification
Position
Peptide
Type
References
109FSRELNVKKENKKVFsumoylation[1]
160GKTRNEVKEESQKREsumoylation[1]
Reference
 [1] SUMO modification is involved in the maintenance of heterochromatin stability in fission yeast.
 Shin JA, Choi ES, Kim HS, Ho JC, Watts FZ, Park SD, Jang YK.
 Mol Cell. 2005 Sep 16;19(6):817-28. [PMID: 16168376] 
Functional Description
 Histone methyltransferase. Catalytic component of the rik1-associated E3 ubiquitin ligase complex that shows ubiquitin ligase activity and is required for histone H3K9 methylation. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting swi6/HP1 to methylated histones which leads to transcriptional silencing within centromeric heterochromatin, telomeric regions and at the silent mating-type loci. 
Sequence Annotation
 DOMAIN 8 69 Chromo.
 DOMAIN 258 325 Pre-SET.
 DOMAIN 328 452 SET.
 DOMAIN 473 489 Post-SET.
 REGION 338 340 S-adenosyl-L-methionine binding (By
 REGION 409 410 S-adenosyl-L-methionine binding (By
 METAL 260 260 Zinc 1.
 METAL 262 262 Zinc 1.
 METAL 268 268 Zinc 2.
 METAL 276 276 Zinc 1.
 METAL 278 278 Zinc 3.
 METAL 307 307 Zinc 2.
 METAL 307 307 Zinc 3.
 METAL 311 311 Zinc 3.
 METAL 313 313 Zinc 2.
 METAL 317 317 Zinc 2.
 METAL 412 412 Zinc 4 (By similarity).
 METAL 477 477 Zinc 4 (By similarity).
 METAL 479 479 Zinc 4 (By similarity).
 METAL 484 484 Zinc 4 (By similarity).
 BINDING 381 381 S-adenosyl-L-methionine (By similarity).  
Keyword
 3D-structure; Chromatin regulator; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Metal-binding; Methyltransferase; Nucleus; Reference proteome; S-adenosyl-L-methionine; Transferase; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 490 AA 
Protein Sequence
MSPKQEEYEV ERIVDEKLDR NGAVKLYRIR WLNYSSRSDT WEPPENLSGC SAVLAEWKRR 60
KRRLKGSNSD SDSPHHASNP HPNSRQKHQH QTSKSVPRSQ RFSRELNVKK ENKKVFSSQT 120
TKRQSRKQST ALTTNDTSII LDDSLHTNSK KLGKTRNEVK EESQKRELVS NSIKEATSPK 180
TSSILTKPRN PSKLDSYTHL SFYEKRELFR KKLREIEGPE VTLVNEVDDE PCPSLDFQFI 240
SQYRLTQGVI PPDPNFQSGC NCSSLGGCDL NNPSRCECLD DLDEPTHFAY DAQGRVRADT 300
GAVIYECNSF CSCSMECPNR VVQRGRTLPL EIFKTKEKGW GVRSLRFAPA GTFITCYLGE 360
VITSAEAAKR DKNYDDDGIT YLFDLDMFDD ASEYTVDAQN YGDVSRFFNH SCSPNIAIYS 420
AVRNHGFRTI YDLAFFAIKD IQPLEELTFD YAGAKDFSPV QSQKSQQNRI SKLRRQCKCG 480
SANCRGWLFG 490 
Gene Ontology
 GO:0043494; C:CLRC ubiquitin ligase complex; IDA:PomBase.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0031934; C:mating-type region heterochromatin; NAS:PomBase.
 GO:0044732; C:mitotic spindle pole body; IDA:PomBase.
 GO:0031618; C:nuclear centromeric heterochromatin; TAS:PomBase.
 GO:0005724; C:nuclear telomeric heterochromatin; NAS:PomBase.
 GO:0046974; F:histone methyltransferase activity (H3-K9 specific); IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0051315; P:attachment of spindle microtubules to kinetochore involved in mitotic sister chromatid segregation; IMP:PomBase.
 GO:0030702; P:chromatin silencing at centromere; IMP:PomBase.
 GO:0000183; P:chromatin silencing at rDNA; IMP:PomBase.
 GO:0030466; P:chromatin silencing at silent mating-type cassette; IMP:PomBase.
 GO:0006348; P:chromatin silencing at telomere; TAS:PomBase.
 GO:0031048; P:chromatin silencing by small RNA; IMP:PomBase.
 GO:0007535; P:donor selection; IMP:PomBase.
 GO:0030989; P:dynein-driven meiotic oscillatory nuclear movement; IGI:PomBase.
 GO:0031507; P:heterochromatin assembly; NAS:PomBase.
 GO:0045141; P:meiotic telomere clustering; IMP:PomBase.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:PomBase.
 GO:0090065; P:regulation of production of siRNA involved in RNA interference; IMP:PomBase.
 GO:0032015; P:regulation of Ran protein signal transduction; TAS:PomBase. 
Interpro
 IPR023780; Chromo_domain.
 IPR000953; Chromo_domain/shadow.
 IPR016197; Chromodomain-like.
 IPR023779; Chromodomain_CS.
 IPR003616; Post-SET_dom.
 IPR007728; Pre-SET_dom.
 IPR003606; Pre-SET_Zn-bd_sub.
 IPR001214; SET_dom. 
Pfam
 PF00385; Chromo
 PF05033; Pre-SET
 PF00856; SET 
SMART
 SM00298; CHROMO
 SM00508; PostSET
 SM00468; PreSET
 SM00317; SET 
PROSITE
 PS00598; CHROMO_1
 PS50013; CHROMO_2
 PS50868; POST_SET
 PS50867; PRE_SET
 PS50280; SET 
PRINTS