CPLM-000465

Genbank Nucleotide ID

Insulin-induced gene 1 protein

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
33	SAAGLAAKVGEMINV	ubiquitination	[1]
156	SHLGEPHKFKREWAS	ubiquitination	[2]
158	LGEPHKFKREWASVM	ubiquitination	[2]
273	LAMGVPEKPHSD***	ubiquitination	[1, 3, 4, 5]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth.

CROSSLNK 156 156 Glycyl lysine isopeptide (Lys-Gly)
CROSSLNK 158 158 Glycyl lysine isopeptide (Lys-Gly)

Alternative splicing; Cholesterol metabolism; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Isopeptide bond; Lipid metabolism; Membrane; Polymorphism; Reference proteome; Steroid metabolism; Sterol metabolism; Transmembrane; Transmembrane helix; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0032937; C:SREBP-SCAP-Insig complex; IDA:UniProtKB.
GO:0008283; P:cell proliferation; TAS:ProtInc.
GO:0006695; P:cholesterol biosynthetic process; IEA:Compara.
GO:0060363; P:cranial suture morphogenesis; IEA:Compara.
GO:0042472; P:inner ear morphogenesis; IEA:Compara.
GO:0042474; P:middle ear morphogenesis; IEA:Compara.
GO:1901303; P:negative regulation of cargo loading into COPII-coated vesicle; IMP:UniProtKB.
GO:0045599; P:negative regulation of fat cell differentiation; IEA:Compara.
GO:0045717; P:negative regulation of fatty acid biosynthetic process; IEA:Compara.
GO:0010894; P:negative regulation of steroid biosynthetic process; IEA:Compara.
GO:0060021; P:palate development; IEA:Compara.
GO:0044281; P:small molecule metabolic process; TAS:Reactome.
GO:0032933; P:SREBP signaling pathway; IDA:UniProtKB.
GO:0006641; P:triglyceride metabolic process; IEA:Compara.

IPR009904; INSIG.
IPR025929; INSIG_fam.