CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030630
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pescadillo homolog 
Protein Synonyms/Alias
 Pescadillo homolog 1, containing BRCT domain (Zebrafish), isoform CRA_b; cDNA FLJ45250 fis, clone BRHIP2008756, highly similar to Pescadillo homolog 1 
Gene Name
 PES1 
Gene Synonyms/Alias
 hCG_2010949 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
136DSESCMEKLAALSASubiquitination[1]
186EAQEKHKKLFEGLKFubiquitination[1]
192KKLFEGLKFFLNREVubiquitination[1, 2, 3]
302YVPPEKLKLLALQRGubiquitination[1, 3, 4]
394QEEESEAKRLAIMMMubiquitination[2, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Nucleus; Reference proteome; Ribosome biogenesis; rRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 449 AA 
Protein Sequence
MCFLFSTFPR TGKCHVQTIQ LCRRLTVEFM HYIIAARALR KVFLSIKGIY YQAEVLGQPI 60
VWITPYAFSH DHPTDVDYRV MATFTEFYTT LLGFVNFRLY QLLNLHYPPK LEGQAQAEAK 120
AGEGTYALDS ESCMEKLAAL SASLARVVVP ATEEEAEVDE FPTDGEMSAQ EEDRRKELEA 180
QEKHKKLFEG LKFFLNREVP REALAFIIRS FGGEVSWDKS LCIGATYDVT DSRITHQIVD 240
RPGQQTSVIG RCYVQPQWVF DSVNARLLLP VAEYFSGVQL PPHLSPFVTE KEGDYVPPEK 300
LKLLALQRGE DPGNLNESEE EEEEDDNNEG DGDEEGENEE EEEDAEAGSE KEEEARLAAL 360
EEQRMEGKKP RVMAGTLKLE DKQRLAQEEE SEAKRLAIMM MKKREKYLYQ KIMFGKRRKI 420
REANKLAEKR KAHDEAVRSE KKAKKARPE 449 
Gene Ontology
 GO:0000793; C:condensed chromosome; IEA:Compara.
 GO:0005730; C:nucleolus; IEA:InterPro.
 GO:0007000; P:nucleolus organization; IEA:Compara.
 GO:0033365; P:protein localization to organelle; IEA:Compara.
 GO:0006364; P:rRNA processing; IEA:UniProtKB-KW. 
Interpro
 IPR001357; BRCT_dom.
 IPR010613; Pescadillo. 
Pfam
 PF00533; BRCT
 PF06732; Pescadillo_N 
SMART
 SM00292; BRCT 
PROSITE
 PS50172; BRCT 
PRINTS