CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000003
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 UBX domain-containing protein 8 
Protein Synonyms/Alias
 Reproduction 8 protein; Rep-8 protein; UBX domain-containing protein 6 
Gene Name
 UBXN8 
Gene Synonyms/Alias
 D8S2298E; REP8; UBXD6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
104RYIENVLKPHQEMKLubiquitination[1, 2]
127QMTGEAWKLSSGHKLubiquitination[3]
133WKLSSGHKLGGDEGTubiquitination[3, 4, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 May play a role in reproduction. 
Sequence Annotation
 DOMAIN 187 263 UBX.
 MOD_RES 167 167 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 270 AA 
Protein Sequence
MASRGVVGIF FLSAVPLVCL ELRRGIPDIG IKDFLLLCGR ILLLLALLTL IISVTTSWLN 60
SFKSPQVYLK EEEEKNEKRQ KLVRKKQQEA QGEKASRYIE NVLKPHQEMK LRKLEERFYQ 120
MTGEAWKLSS GHKLGGDEGT SQTSFETSNR EAAKSQNLPK PLTEFPSPAE QPTCKEIPDL 180
PEEPSQTAEE VVTVALRCPS GNVLRRRFLK SYSSQVLFDW MTRIGYHISL YSLSTSFPRR 240
PLAVEGGQSL EDIGITVDTV LILEEKEQTN 270 
Gene Ontology
 GO:0007338; P:single fertilization; TAS:ProtInc. 
Interpro
 IPR001012; UBX.
 IPR017247; UBX_Rep6. 
Pfam
 PF00789; UBX 
SMART
 SM00166; UBX 
PROSITE
 PS50033; UBX 
PRINTS