CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018004
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Minor histocompatibility antigen H13 
Protein Synonyms/Alias
 Intramembrane protease 1; IMP-1; IMPAS-1; hIMP1; Presenilin-like protein 3; Signal peptide peptidase 
Gene Name
 HM13 
Gene Synonyms/Alias
 H13; IMP1; PSL3; SPP; MSTP086 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
61SVRCARGKNASDMPEubiquitination[1, 2, 3]
241KSFEAPIKLVFPQDLubiquitination[2, 3, 4]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Catalyzes intramembrane proteolysis of some signal peptides after they have been cleaved from a preprotein, resulting in the release of the fragment from the ER membrane into the cytoplasm. Required to generate lymphocyte cell surface (HLA-E) epitopes derived from MHC class I signal peptides. May play a role in graft rejection (By similarity). May be necessary for the removal of the signal peptide that remains attached to the hepatitis C virus core protein after the initial proteolytic processing of the polyprotein. Involved in the intramembrane cleavage of the integral membrane protein PSEN1. 
Sequence Annotation
 MOTIF 317 319 PAL.
 ACT_SITE 219 219 By similarity.
 ACT_SITE 265 265 By similarity.
 CARBOHYD 10 10 N-linked (GlcNAc...).
 CARBOHYD 20 20 N-linked (GlcNAc...).  
Keyword
 Alternative splicing; Cell membrane; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Hydrolase; Membrane; Polymorphism; Protease; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 377 AA 
Protein Sequence
MDSALSDPHN GSAEAGGPTN STTRPPSTPE GIALAYGSLL LMALLPIFFG ALRSVRCARG 60
KNASDMPETI TSRDAARFPI IASCTLLGLY LFFKIFSQEY INLLLSMYFF VLGILALSHT 120
ISPFMNKFFP ASFPNRQYQL LFTQGSGENK EEIINYEFDT KDLVCLGLSS IVGVWYLLRK 180
HWIANNLFGL AFSLNGVELL HLNNVSTGCI LLGGLFIYDV FWVFGTNVMV TVAKSFEAPI 240
KLVFPQDLLE KGLEANNFAM LGLGDVVIPG IFIALLLRFD ISLKKNTHTY FYTSFAAYIF 300
GLGLTIFIMH IFKHAQPALL YLVPACIGFP VLVALAKGEV TEMFSYESSA EILPHTPRLT 360
HFPTVSGSPA SLADSMQQKL AGPRRRRPQN PSAIYEESNP KDPAAVTESK EGTEASASKG 420
LEKKEK 426 
Gene Ontology
 GO:0009986; C:cell surface; ISS:UniProtKB.
 GO:0071458; C:integral to cytosolic side of endoplasmic reticulum membrane; IDA:UniProtKB.
 GO:0071556; C:integral to lumenal side of endoplasmic reticulum membrane; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005791; C:rough endoplasmic reticulum; IDA:UniProtKB.
 GO:0042500; F:aspartic endopeptidase activity, intramembrane cleaving; IMP:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0033619; P:membrane protein proteolysis; IDA:UniProtKB. 
Interpro
 IPR006639; Peptidase_A22.
 IPR007369; Peptidase_A22B_SPP. 
Pfam
 PF04258; Peptidase_A22B 
SMART
 SM00730; PSN 
PROSITE
  
PRINTS