CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002859
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Replicative DNA helicase 
Protein Synonyms/Alias
  
Gene Name
 dnaB 
Gene Synonyms/Alias
 STM4246 
Created Date
 July 27, 2013 
Organism
 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 
NCBI Taxa ID
 99287 
Lysine Modification
Position
Peptide
Type
References
175IAENRANKDEGPKSIacetylation[1]
180ANKDEGPKSIDQILDacetylation[1]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins. 
Sequence Annotation
 DOMAIN 200 467 SF4 helicase.
 NP_BIND 231 238 ATP (Probable).  
Keyword
 ATP-binding; Complete proteome; DNA replication; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Primosome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 471 AA 
Protein Sequence
MAGNKPFNKP QTDARDRDPQ VAGIKVPPHS IEAEQSVLGG LMLDNERWDD VAERVVAEDF 60
YTRPHRHIFT EMGRLQESGS PIDLITLAES LERQGQLDSV GGFAYLAELS KNTPSAANIS 120
AYADIVRERA VVRDMIAVAH EIADAGYDPQ GRNSDELLDL AESRVFQIAE NRANKDEGPK 180
SIDQILDATV ARIEQLFQQP HDGVTGVDTG YQDLNKKTAG LQRSDLIIVA ARPSMGKTTF 240
AMNLCENAAM LQDKPVLIFS LEMPGEQIMM RMLASLSRVD QTRIRTGQLD DEDWARISGT 300
MGILLEKRNM YIDDSSGLTP TEVRSRARRI FREHGGLSLI MIDYLQLMRV PSLSDNRTLE 360
IAEISRSLKA LAKELQVPVV ALSQLNRSLE QRADKRPVNS DLRESGSIEQ DADLIMFIYR 420
DEVYHENSDL KGIAEIIIGK QRNGPIGTVR LTFNGQWSRF DNYAGPQYDD E 471 
Gene Ontology
 GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003678; F:DNA helicase activity; IEA:InterPro.
 GO:0032508; P:DNA duplex unwinding; IEA:GOC.
 GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR007692; DNA_helicase_DnaB.
 IPR007694; DNA_helicase_DnaB-like_C.
 IPR007693; DNA_helicase_DnaB-like_N.
 IPR016136; DNA_helicase_N/primase_C.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00772; DnaB
 PF03796; DnaB_C 
SMART
 SM00382; AAA 
PROSITE
 PS51199; SF4_HELICASE 
PRINTS