CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023269
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Solute carrier family 12 member 4 
Protein Synonyms/Alias
 Electroneutral potassium-chloride cotransporter 1; Erythroid K-Cl cotransporter 1; hKCC1 
Gene Name
 SLC12A4 
Gene Synonyms/Alias
 KCC1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
78EELDIRPKVSSLLGKubiquitination[1, 2]
85KVSSLLGKLVSYTNLubiquitination[3, 4, 5, 6]
97TNLTQGAKEHEEAESubiquitination[1]
535RLLQAIAKDNIIPFLubiquitination[1, 3, 7]
659IEYQGAEKEWGDGIRubiquitination[1]
707LDEDLHVKYPRLLTFubiquitination[3]
988QMTWTRDKYMTETWDubiquitination[1]
1010FRELVHIKPDQSNVRubiquitination[1, 4, 7]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia (By similarity). Isoform 4 has no transport activity. 
Sequence Annotation
 MOD_RES 47 47 Phosphoserine (By similarity).
 MOD_RES 51 51 Phosphoserine.
 MOD_RES 967 967 Phosphoserine.
 MOD_RES 983 983 Phosphothreonine (By similarity).
 CARBOHYD 245 245 N-linked (GlcNAc...) (Potential).
 CARBOHYD 312 312 N-linked (GlcNAc...) (Potential).
 CARBOHYD 331 331 N-linked (GlcNAc...) (Potential).
 CARBOHYD 347 347 N-linked (GlcNAc...) (Potential).
 CARBOHYD 439 439 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Complete proteome; Glycoprotein; Ion transport; Membrane; Phosphoprotein; Potassium; Potassium transport; Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1085 AA 
Protein Sequence
MPHFTVVPVD GPRRGDYDNL EGLSWVDYGE RAELDDSDGH GNHRESSPFL SPLEASRGID 60
YYDRNLALFE EELDIRPKVS SLLGKLVSYT NLTQGAKEHE EAESGEGTRR RAAEAPSMGT 120
LMGVYLPCLQ NIFGVILFLR LTWMVGTAGV LQALLIVLIC CCCTLLTAIS MSAIATNGVV 180
PAGGSYFMIS RSLGPEFGGA VGLCFYLGTT FAAAMYILGA IEILLTYIAP PAAIFYPSGA 240
HDTSNATLNN MRVYGTIFLT FMTLVVFVGV KYVNKFASLF LACVIISILS IYAGGIKSIF 300
DPPVFPVCML GNRTLSRDQF DICAKTAVVD NETVATQLWS FFCHSPNLTT DSCDPYFMLN 360
NVTEIPGIPG AAAGVLQENL WSAYLEKGDI VEKHGLPSAD APSLKESLPL YVVADIATSF 420
TVLVGIFFPS VTGIMAGSNR SGDLRDAQKS IPVGTILAII TTSLVYFSSV VLFGACIEGV 480
VLRDKYGDGV SRNLVVGTLA WPSPWVIVIG SFFSTCGAGL QSLTGAPRLL QAIAKDNIIP 540
FLRVFGHGKV NGEPTWALLL TALIAELGIL IASLDMVAPI LSMFFLMCYL FVNLACAVQT 600
LLRTPNWRPR FKYYHWALSF LGMSLCLALM FVSSWYYALV AMLIAGMIYK YIEYQGAEKE 660
WGDGIRGLSL SAARYALLRL EEGPPHTKNW RPQLLVLLKL DEDLHVKYPR LLTFASQLKA 720
GKGLTIVGSV IQGSFLESYG EAQAAEQTIK NMMEIEKVKG FCQVVVASKV REGLAHLIQS 780
CGLGGMRHNS VVLGWPYGWR QSEDPRAWKT FIDTVRCTTA AHLALLVPKN IAFYPSNHER 840
YLEGHIDVWW IVHDGGMLML LPFLLRQHKV WRKCRMRIFT VAQMDDNSIQ MKKDLAVFLY 900
HLRLEAEVEV VEMHNSDISA YTYERTLMME QRSQMLRQMR LTKTEREREA QLVKDRHSAL 960
RLESLYSDEE DESAVGADKI QMTWTRDKYM TETWDPSHAP DNFRELVHIK PDQSNVRRMH 1020
TAVKLNEVIV TRSHDARLVL LNMPGPPRNS EGDENYMEFL EVLTEGLERV LLVRGGGREV 1080
ITIYS 1085 
Gene Ontology
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0015379; F:potassium:chloride symporter activity; TAS:ProtInc.
 GO:0006884; P:cell volume homeostasis; TAS:ProtInc.
 GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW. 
Interpro
 IPR004841; AA-permease_dom.
 IPR000622; K/Cl_cotranspt1.
 IPR018491; K/Cl_cotranspt_1/3.
 IPR000076; KCL_cotranspt.
 IPR004842; Na/K/Cl_cotransptS. 
Pfam
 PF00324; AA_permease
 PF03522; KCl_Cotrans_1 
SMART
  
PROSITE
  
PRINTS
 PR01081; KCLTRNSPORT.
 PR01082; KCLTRNSPORT1.