CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009511
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytoplasmic trehalase 
Protein Synonyms/Alias
 Alpha,alpha-trehalase; Alpha,alpha-trehalose glucohydrolase 
Gene Name
 treF 
Gene Synonyms/Alias
 b3519; JW3487 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
369IANISALKGEKETEAacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Hydrolyzes trehalose to glucose. Could be involved, in cells returning to low osmolarity conditions, in the utilization of the accumulated cytoplasmic trehalose, which was synthesized in response to high osmolarity. 
Sequence Annotation
 REGION 175 176 Substrate binding (By similarity).
 REGION 221 223 Substrate binding (By similarity).
 REGION 292 294 Substrate binding (By similarity).
 ACT_SITE 326 326 Proton donor/acceptor (By similarity).
 ACT_SITE 509 509 Proton donor/acceptor (By similarity).
 BINDING 168 168 Substrate (By similarity).
 BINDING 212 212 Substrate (By similarity).
 BINDING 324 324 Substrate; via carbonyl oxygen (By
 BINDING 525 525 Substrate (By similarity).  
Keyword
 Complete proteome; Cytoplasm; Direct protein sequencing; Glycosidase; Hydrolase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 549 AA 
Protein Sequence
MLNQKIQNPN PDELMIEVDL CYELDPYELK LDEMIEAEPE PEMIEGLPAS DALTPADRYL 60
ELFEHVQSAK IFPDSKTFPD CAPKMDPLDI LIRYRKVRRH RDFDLRKFVE NHFWLPEVYS 120
SEYVSDPQNS LKEHIDQLWP VLTREPQDHI PWSSLLALPQ SYIVPGGRFS ETYYWDSYFT 180
MLGLAESGRE DLLKCMADNF AWMIENYGHI PNGNRTYYLS RSQPPVFALM VELFEEDGVR 240
GARRYLDHLK MEYAFWMDGA ESLIPNQAYR HVVRMPDGSL LNRYWDDRDT PRDESWLEDV 300
ETAKHSGRPP NEVYRDLRAG AASGWDYSSR WLRDTGRLAS IRTTQFIPID LNAFLFKLES 360
AIANISALKG EKETEALFRQ KASARRDAVN RYLWDDENGI YRDYDWRREQ LALFSAAAIV 420
PLYVGMANHE QADRLANAVR SRLLTPGGIL ASEYETGEQW DKPNGWAPLQ WMAIQGFKMY 480
GDDLLGDEIA RSWLKTVNQF YLEQHKLIEK YHIADGVPRE GGGGEYPLQD GFGWTNGVVR 540
RLIGLYGEP 549 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004555; F:alpha,alpha-trehalase activity; IDA:EcoCyc.
 GO:0071474; P:cellular hyperosmotic response; IEP:EcoCyc.
 GO:0005993; P:trehalose catabolic process; IMP:EcoCyc. 
Interpro
 IPR008928; 6-hairpin_glycosidase-like.
 IPR023715; Cyt_trehalase.
 IPR001661; Glyco_hydro_37.
 IPR018232; Glyco_hydro_37_CS. 
Pfam
 PF01204; Trehalase 
SMART
  
PROSITE
 PS00927; TREHALASE_1
 PS00928; TREHALASE_2 
PRINTS
 PR00744; GLHYDRLASE37.