| Tag | Content |
|---|
| CPLM ID | CPLM-004166 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Chromatin assembly factor 1 subunit p50 |
Protein Synonyms/Alias | CAF-1 p50 subunit; IRA1 multicopy suppressor; Protein MSI1 |
Gene Name | MSI1 |
Gene Synonyms/Alias | CAC3; YBR195C; YBR1405 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 6 | **MNQCAKDITHEAS | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Acts as a component of chromatin assembly factor 1 (CAF- 1), which assembles histone octamers onto replicating DNA in vitro. It performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. p50 and p60 form complexes with newly synthesized histones H3 and acetylated H4 in cell extracts (By similarity). Independently, MSI1 is involved in regulation of the RAS/cAMP pathway via sequestration of NPR1. |
Sequence Annotation | REPEAT 127 158 WD 1.
REPEAT 198 238 WD 2.
REPEAT 249 289 WD 3.
REPEAT 294 334 WD 4.
REPEAT 338 379 WD 5.
REPEAT 382 421 WD 6. |
Keyword | cAMP; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Reference proteome; Repeat; WD repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 422 AA |
Protein Sequence | MNQCAKDITH EASSIPIDLQ ERYSHWKKNT KLLYDYLNTN STKWPSLTCQ FFPDLDTTSD 60
EHRILLSSFT SSQKPEDETI YISKISTLGH IKWSSLNNFD MDEMEFKPEN STRFPSKHLV 120
NDISIFFPNG ECNRARYLPQ NPDIIAGASS DGAIYIFDRT KHGSTRIRQS KISHPFETKL 180
FGSHGVIQDV EAMDTSSADI NEATSLAWNL QQEALLLSSH SNGQVQVWDI KQYSHENPII 240
DLPLVSINSD GTAVNDVTWM PTHDSLFAAC TEGNAVSLLD LRTKKEKLQS NREKHDGGVN 300
SCRFNYKNSL ILASADSNGR LNLWDIRNMN KSPIATMEHG TSVSTLEWSP NFDTVLATAG 360
QEDGLVKLWD TSCEETIFTH GGHMLGVNDI SWDAHDPWLM CSVANDNSVH IWKPAGNLVG 420
HS 422 |
Gene Ontology | |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |