UniProt Accession

 RLMN_ECOLI; P36979 

Genbank Protein ID

 U02965; U00096; AP009048 

Genbank Nucleotide ID

 AAA21359.1; AAC75570.1; BAA16404.1 

Protein Name

 Dual-specificity RNA methyltransferase RlmN 

Protein Synonyms/Alias

 23S rRNA (adenine(2503)-C(2))-methyltransferase; 23S rRNA m2A2503 methyltransferase; Ribosomal RNA large subunit methyltransferase N; tRNA (adenine(37)-C(2))-methyltransferase; tRNA m2A37 methyltransferase 

Gene Name

 rlmN 

Gene Synonyms/Alias

 yfgB; b2517; JW2501 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
33	QQMREFFKDLGEKPF	acetylation	[1]
38	FFKDLGEKPFRADQV	acetylation	[1]
382	QGEAIDIKAV*****	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity. Unmodified tRNA is not a suitable substrate for RlmN, which suggests that RlmN works in a late step during tRNA maturation. 

Sequence Annotation

 REGION 179 180 S-adenosyl-L-methionine binding.
 REGION 233 235 S-adenosyl-L-methionine binding.
 ACT_SITE 105 105 Proton acceptor (Potential).
 ACT_SITE 355 355 S-methylcysteine intermediate.
 METAL 125 125 Iron-sulfur (4Fe-4S-S-AdoMet).
 METAL 129 129 Iron-sulfur (4Fe-4S-S-AdoMet).
 METAL 132 132 Iron-sulfur (4Fe-4S-S-AdoMet).
 BINDING 211 211 S-adenosyl-L-methionine.
 BINDING 312 312 S-adenosyl-L-methionine; via amide
 DISULFID 118 355 (transient) (Probable).  

Keyword

 3D-structure; 4Fe-4S; Antibiotic resistance; Complete proteome; Cytoplasm; Disulfide bond; Iron; Iron-sulfur; Metal-binding; Methyltransferase; Reference proteome; rRNA processing; S-adenosyl-L-methionine; Transferase; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 384 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0070040; F:rRNA (adenine-C2-)-methyltransferase activity; IDA:EcoCyc.
 GO:0019843; F:rRNA binding; IEA:HAMAP.
 GO:0002935; F:tRNA (adenine-C2-)-methyltransferase activity; IDA:UniProtKB.
 GO:0000049; F:tRNA binding; IDA:UniProtKB.
 GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
 GO:0070475; P:rRNA base methylation; IMP:EcoCyc. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR006638; Elp3/MiaB/NifB.
 IPR027492; RNA_MTrfase_RlmN.
 IPR004383; rRNA_lsu_MTrfase_RlmN/Cfr.
 IPR007197; rSAM. 

Pfam

 PF04055; Radical_SAM 

SMART

 SM00729; Elp3 

PROSITE

  

PRINTS