CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009048
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Excitatory amino acid transporter 1 
Protein Synonyms/Alias
 Glial high affinity glutamate transporter; High-affinity neuronal glutamate transporter; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3 
Gene Name
 Slc1a3 
Gene Synonyms/Alias
 Eaat1; Gmt1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
42SLTKEDVKSYLFRNAubiquitination[1]
384EENNGVDKRITRFVLubiquitination[1]
503HLSRHELKNRDVEMGubiquitination[1]
520VIEENEMKKPYQLIAubiquitination[1]
521IEENEMKKPYQLIAQubiquitination[1]
534AQDNEPEKPVADSETubiquitination[1]
542PVADSETKM******ubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. 
Sequence Annotation
 MOD_RES 512 512 Phosphoserine.
 CARBOHYD 206 206 N-linked (GlcNAc...).
 CARBOHYD 215 215 N-linked (GlcNAc...); atypical.
 CARBOHYD 216 216 N-linked (GlcNAc...).  
Keyword
 Complete proteome; Direct protein sequencing; Glycoprotein; Membrane; Phosphoprotein; Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 543 AA 
Protein Sequence
MTKSNGEEPR MGGRMERLQQ GVRKRTLLAK KKVQSLTKED VKSYLFRNAF VLLTVTAVIV 60
GTILGFALRP YKMSYREVKY FSFPGELLMR MLQMLVLPLI ISSLVTGMAA LDSKASGKMG 120
MRAVVYYMTT TIIAVVIGII IVIIIHPGKG TKENMYREGK IVQVTAADAF LDLIRNMFPP 180
NLVEACFKQF KTSYEKRSFK VPIQSNETLL GAVINNVSEA METLTRIREE MVPVPGSVNG 240
VNALGLVVFS MCFGFVIGNM KEQGQALREF FDSLNEAIMR LVAVIMWYAP LGILFLIAGK 300
IVEMEDMGVI GGQLAMYTVT VIVGLLIHAV IVLPLLYFLV TRKNPWVFIG GLLQALITAL 360
GTSSSSATLP ITFKCLEENN GVDKRITRFV LPVGATINMD GTALYEALAA IFIAQVNNFD 420
LNFGQIITIS ITATAASIGA AGIPQAGLVT MVIVLTSVGL PTDDITLIIA VDWFLDRLRT 480
TTNVLGDSLG AGIVEHLSRH ELKNRDVEMG NSVIEENEMK KPYQLIAQDN EPEKPVADSE 540
TKM 543 
Gene Ontology
 GO:0042995; C:cell projection; IDA:MGI.
 GO:0009986; C:cell surface; IDA:MGI.
 GO:0043205; C:fibril; IDA:MGI.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0043025; C:neuronal cell body; IDA:MGI.
 GO:0005886; C:plasma membrane; IDA:MGI.
 GO:0016595; F:glutamate binding; IDA:MGI.
 GO:0005314; F:high-affinity glutamate transmembrane transporter activity; IDA:MGI.
 GO:0017153; F:sodium:dicarboxylate symporter activity; IEA:InterPro.
 GO:0031223; P:auditory behavior; IMP:MGI.
 GO:0048667; P:cell morphogenesis involved in neuron differentiation; IMP:MGI.
 GO:0021545; P:cranial nerve development; IMP:MGI.
 GO:0070779; P:D-aspartate import; IEA:Compara.
 GO:0009449; P:gamma-aminobutyric acid biosynthetic process; IMP:MGI.
 GO:0006537; P:glutamate biosynthetic process; IMP:MGI.
 GO:0051938; P:L-glutamate import; IMP:MGI.
 GO:0050885; P:neuromuscular process controlling balance; IMP:MGI.
 GO:0050806; P:positive regulation of synaptic transmission; IMP:MGI.
 GO:0043200; P:response to amino acid stimulus; ISO:MGI.
 GO:0046677; P:response to antibiotic; IMP:MGI.
 GO:0042493; P:response to drug; IMP:MGI.
 GO:0010035; P:response to inorganic substance; ISO:MGI.
 GO:0009416; P:response to light stimulus; IMP:MGI.
 GO:0009611; P:response to wounding; IMP:MGI.
 GO:0007605; P:sensory perception of sound; IMP:MGI. 
Interpro
 IPR001991; Na-dicarboxylate_symporter.
 IPR018107; Na-dicarboxylate_symporter_CS. 
Pfam
 PF00375; SDF 
SMART
  
PROSITE
 PS00713; NA_DICARBOXYL_SYMP_1
 PS00714; NA_DICARBOXYL_SYMP_2 
PRINTS
 PR00173; EDTRNSPORT.