UniProt Accession

 PAR16_HUMAN; Q8N5Y8; Q6PK64; Q9NX03 

Genbank Protein ID

 AK000516; BC006389; BC031074 

Genbank Nucleotide ID

 BAA91222.1; AAH06389.1; AAH31074.1 

Protein Name

 Mono [ADP-ribose] polymerase PARP16 

Protein Synonyms/Alias

 ADP-ribosyltransferase diphtheria toxin-like 15; ARTD15; Poly [ADP-ribose] polymerase 16; PARP-16 

Gene Name

 PARP16 

Gene Synonyms/Alias

 C15orf30 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
35	ASALQSYKRDSVLRP	ubiquitination	[1, 2]
142	NAKFYETKGERDLIY	ubiquitination	[3]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Mono-ADP-ribosyltransferase targeting the karyopherin KPNB1. Plays a role in unfolded protein response (UPR), by ADP- ribosylating and activating EIF2AK3 and ERN1, two important UPR effectors. 

Sequence Annotation

 DOMAIN 5 91 PARP alpha-helical.
 DOMAIN 94 279 PARP catalytic.
 BINDING 152 152 NAD.
 BINDING 182 182 NAD.
 BINDING 254 254 NAD; may stabilize a reaction  

Keyword

 3D-structure; ADP-ribosylation; Alternative splicing; Complete proteome; Endoplasmic reticulum; Glycosyltransferase; Membrane; NAD; Nucleus; Polymorphism; Reference proteome; Transferase; Transmembrane; Transmembrane helix; Unfolded protein response. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 322 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0003956; F:NAD(P)+-protein-arginine ADP-ribosyltransferase activity; IEA:EC.
 GO:0003950; F:NAD+ ADP-ribosyltransferase activity; IEA:InterPro.
 GO:0006986; P:response to unfolded protein; IEA:UniProtKB-KW. 

Interpro

 IPR012317; Poly(ADP-ribose)pol_cat_dom. 

Pfam

 PF00644; PARP 

SMART

  

PROSITE

 PS51060; PARP_ALPHA_HD
 PS51059; PARP_CATALYTIC 

PRINTS