UniProt Accession

 UBA3_RAT; Q99MI7 

Genbank Protein ID

 AF336829; BC081743 

Genbank Nucleotide ID

 AAK21298.1; AAH81743.1 

Protein Name

 NEDD8-activating enzyme E1 catalytic subunit 

Protein Synonyms/Alias

 NEDD8-activating enzyme E1C; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3; Ubiquitin-activating enzyme 3 

Gene Name

 Uba3 

Gene Synonyms/Alias

 Ube1c 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
307	RLTQGVVKRIIPAVA	ubiquitination	[1]
409	ITATLEGKNRTLYLQ	acetylation	[2]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. 

Sequence Annotation

 NP_BIND 100 124 ATP (By similarity).
 NP_BIND 148 171 ATP (By similarity).
 REGION 53 70 Interaction with UBE2M N-terminus (By
 REGION 157 161 Interaction with UBE2M N-terminus (By
 REGION 192 217 Interaction with UBE2M N-terminus (By
 REGION 227 229 Interaction with NEDD8 (By similarity).
 REGION 242 248 Interaction with NAE1 (By similarity).
 REGION 292 295 Interaction with NAE1 (By similarity).
 REGION 331 338 Interaction with UBE2M N-terminus (By
 REGION 352 357 Interaction with NEDD8 (By similarity).
 REGION 368 462 Interaction with UBE2M core domain (By
 ACT_SITE 237 237 Glycyl thioester intermediate (By  

Keyword

 ATP-binding; Cell cycle; Complete proteome; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 462 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IEA:Compara.
 GO:0016881; F:acid-amino acid ligase activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0019781; F:NEDD8 activating enzyme activity; IDA:RGD.
 GO:0046982; F:protein heterodimerization activity; IMP:RGD.
 GO:0007113; P:endomitotic cell cycle; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:RGD.
 GO:0045116; P:protein neddylation; TAS:RGD.
 GO:0051726; P:regulation of cell cycle; IEA:Compara. 

Interpro

 IPR014929; E2_binding.
 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd.
 IPR023318; Ub_act_enz_dom_a.
 IPR000127; UBact_repeat.
 IPR019572; Ubiquitin-activating_enzyme.
 IPR018074; UBQ-activ_enz_E1_AS. 

Pfam

 PF08825; E2_bind
 PF00899; ThiF
 PF10585; UBA_e1_thiolCys
 PF02134; UBACT 

SMART

  

PROSITE

 PS00536; UBIQUITIN_ACTIVAT_1
 PS00865; UBIQUITIN_ACTIVAT_2 

PRINTS