CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012524
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rab GTPase-binding effector protein 1 
Protein Synonyms/Alias
 Rabaptin-4; Rabaptin-5; Rabaptin-5alpha; Renal carcinoma antigen NY-REN-17 
Gene Name
 RABEP1 
Gene Synonyms/Alias
 RAB5EP; RABPT5; RABPT5A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
107QEAIDEVKRQWREEVubiquitination[1]
282QLKHTWQKANDQFLEacetylation[2]
520TEWNLLQKEVHNAGNubiquitination[3, 4]
647EELVRLQKDNDSLQGubiquitination[1]
852RAILNDTKLTDINQLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Rab effector protein acting as linker between gamma- adaptin, RAB4A and RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A. 
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 282 282 N6-acetyllysine.
 MOD_RES 407 407 Phosphoserine.
 MOD_RES 410 410 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Apoptosis; Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Direct protein sequencing; Endocytosis; Endosome; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 862 AA 
Protein Sequence
MAQPGPASQP DVSLQQRVAE LEKINAEFLR AQQQLEQEFN QKRAKFKELY LAKEEDLKRQ 60
NAVLQAAQDD LGHLRTQLWE AQAEMENIKA IATVSENTKQ EAIDEVKRQW REEVASLQAV 120
MKETVRDYEH QFHLRLEQER TQWAQYRESA EREIADLRRR LSEGQEEENL ENEMKKAQED 180
AEKLRSVVMP MEKEIAALKD KLTEAEDKIK ELEASKVKEL NHYLEAEKSC RTDLEMYVAV 240
LNTQKSVLQE DAEKLRKELH EVCHLLEQER QQHNQLKHTW QKANDQFLES QRLLMRDMQR 300
MEIVLTSEQL RQVEELKKKD QEDDEQQRLN KRKDHKKADV EEEIKIPVVC ALTQEESSAQ 360
LSNEEEHLDS TRGSVHSLDA GLLLPSGDPF SKSDNDMFKD GLRRAQSTDS LGTSGSLQSK 420
ALGYNYKAKS AGNLDESDFG PLVGADSVSE NFDTASLGSL QMPSGFMLTK DQERAIKAMT 480
PEQEETASLL SSVTQGMESA YVSPSGYRLV SETEWNLLQK EVHNAGNKLG RRCDMCSNYE 540
KQLQGIQIQE AETRDQVKKL QLMLRQANDQ LEKTMKDKQE LEDFIKQSSE DSSHQISALV 600
LRAQASEILL EELQQGLSQA KRDVQEQMAV LMQSREQVSE ELVRLQKDND SLQGKHSLHV 660
SLQQAEDFIL PDTTEALREL VLKYREDIIN VRTAADHVEE KLKAEILFLK EQIQAEQCLK 720
ENLEETLQLE IENCKEEIAS ISSLKAELER IKVEKGQLES TLREKSQQLE SLQEIKISLE 780
EQLKKETAAK ATVEQLMFEE KNKAQRLQTE LDVSEQVQRD FVKLSQTLQV QLERIRQADS 840
LERIRAILND TKLTDINQLP ET 862 
Gene Ontology
 GO:0005769; C:early endosome; TAS:ProtInc.
 GO:0030139; C:endocytic vesicle; IDA:UniProtKB.
 GO:0055037; C:recycling endosome; IEA:UniProtKB-SubCell.
 GO:0005096; F:GTPase activator activity; IEA:InterPro.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0006944; P:cellular membrane fusion; TAS:ProtInc.
 GO:0006897; P:endocytosis; TAS:ProtInc.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR003914; Rabaptin.
 IPR018514; Rabaptin_coiled-coil.
 IPR015390; Rabaptin_Rab5-bd_dom. 
Pfam
 PF09311; Rab5-bind
 PF03528; Rabaptin 
SMART
  
PROSITE
  
PRINTS
 PR01432; RABAPTIN.