CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022505
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SH3 domain-binding protein 4 
Protein Synonyms/Alias
 EH-binding protein 10; Transferrin receptor-trafficking protein 
Gene Name
 SH3BP4 
Gene Synonyms/Alias
 BOG25; EHB10; TTP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
270SSFFTGLKSPAPEQFubiquitination[1]
657DRPVSSLKFGKLLKTubiquitination[2, 3]
678NHYLLEYKKGDGIALubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 May function in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin- mediated endocytosis. Alternatively, may acts as a negative regulator of the amino acid-induced TOR signaling by inhibiting the formation of active Rag GTPase complexes. Preferentially binds inactive Rag GTPase complexes and prevents their interaction with the mTORC1 complex inhibiting its relocalization to lysosomes and its activation. Thereby, may indirectly regulate cell growth, proliferation and autophagy. 
Sequence Annotation
 DOMAIN 55 114 SH3.
 MOD_RES 131 131 Phosphoserine.
 MOD_RES 246 246 Phosphoserine.
 MOD_RES 637 637 Phosphoserine.  
Keyword
 Alternative splicing; Coated pit; Complete proteome; Cytoplasmic vesicle; Endocytosis; Membrane; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 963 AA 
Protein Sequence
MAAQRIRAAN SNGLPRCKSE GTLIDLSEGF SETSFNDIKV PSPSALLVDN PTPFGNAKEV 60
IAIKDYCPTN FTTLKFSKGD HLYVLDTSGG EWWYAHNTTE MGYIPSSYVQ PLNYRNSTLS 120
DSGMIDNLPD SPDEVAKELE LLGGWTDDKK VPGRMYSNNP FWNGVQTNPF LNGNVPVMPS 180
LDELNPKSTV DLLLFDAGTS SFTESSSATT NSTGNIFDEL PVTNGLHAEP PVRRDNPFFR 240
SKRSYSLSEL SVLQAKSDAP TSSSFFTGLK SPAPEQFQSR EDFRTAWLNH RKLARSCHDL 300
DLLGQSPGWG QTQAVETNIV CKLDSSGGAV QLPDTSISIH VPEGHVAPGE TQQISMKALL 360
DPPLELNSDR SCSISPVLEV KLSNLEVKTS IILEMKVSAE IKNDLFSKST VGLQCLRSDS 420
KEGPYVSVPL NCSCGDTVQA QLHNLEPCMY VAVVAHGPSI LYPSTVWDFI NKKVTVGLYG 480
PKHIHPSFKT VVTIFGHDCA PKTLLVSEVT RQAPNPAPVA LQLWGKHQFV LSRPQDLKVC 540
MFSNMTNYEV KASEQAKVVR GFQLKLGKVS RLIFPITSQN PNELSDFTLR VQVKDDQEAI 600
LTQFCVQTPQ PPPKSAIKPS GQRRFLKKNE VGKIILSPFA TTTKYPTFQD RPVSSLKFGK 660
LLKTVVRQNK NHYLLEYKKG DGIALLSEER VRLRGQLWTK EWYIGYYQGR VGLVHTKNVL 720
VVGRARPSLC SGPELSTSVL LEQILRPCKF LTYIYASVRT LLMENISSWR SFADALGYVN 780
LPLTFFCRAE LDSEPERVAS VLEKLKEDCN NTENKERKSF QKELVMALLK MDCQGLVVRL 840
IQDFVLLTTA VEVAQRWREL AEKLAKVSKQ QMDAYESPHR DRNGVVDSEA MWKPAYDFLL 900
TWSHQIGDSY RDVIQELHLG LDKMKNPITK RWKHLTGTLI LVNSLDVLRA AAFSPADQDD 960
FVI 963 
Gene Ontology
 GO:0030136; C:clathrin-coated vesicle; IEA:UniProtKB-SubCell.
 GO:0005905; C:coated pit; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005092; F:GDP-dissociation inhibitor activity; IMP:UniProtKB.
 GO:0071230; P:cellular response to amino acid stimulus; IMP:UniProtKB.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0030308; P:negative regulation of cell growth; IMP:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IMP:UniProtKB.
 GO:0034260; P:negative regulation of GTPase activity; IDA:UniProtKB.
 GO:0032007; P:negative regulation of TOR signaling cascade; IMP:UniProtKB.
 GO:0010508; P:positive regulation of autophagy; IMP:UniProtKB.
 GO:0061462; P:protein localization to lysosome; IMP:UniProtKB. 
Interpro
 IPR011511; SH3_2.
 IPR001452; SH3_domain.
 IPR000906; ZU5. 
Pfam
 PF00018; SH3_1
 PF07653; SH3_2
 PF00791; ZU5 
SMART
 SM00326; SH3 
PROSITE
 PS50002; SH3 
PRINTS