UniProt Accession

 TBA1B_MOUSE; P05213; Q3TY23; Q3U8B1; Q3UAW8; Q4KMW2 

Genbank Protein ID

 M13446; AK075955; AK137885; AK150128; AK150179; AK150968; AK151199; AK151809; AK152298; AK153064; AK153254; AK158958; AK164428; AK168770; AK169075; AK169092; AK169130; AK169665; BC002219; BC008117; BC063777; BC083120; BC098321; BC108337; BC108394; M28727 

Genbank Nucleotide ID

 AAA40500.1; BAC36080.1; BAE23512.1; BAE29327.1; BAE29362.1; BAE29999.1; BAE30196.1; BAE30708.1; BAE31107.1; BAE31690.1; BAE31845.1; BAE34741.1; BAE37783.1; BAE40606.1; BAE40860.1; BAE40875.1; BAE40909.1; BAE41287.1; AAH02219.1; AAH08117.1; AAH63777.1; AAH83120.1; AAH98321.1; AAI08338.1; AAI08395.1; AAA40507.1 

Protein Name

 Tubulin alpha-1B chain 

Protein Synonyms/Alias

 Alpha-tubulin 2; Alpha-tubulin isotype M-alpha-2; Tubulin alpha-2 chain 

Gene Name

 Tuba1b 

Gene Synonyms/Alias

 Tuba2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
40	DGQMPSDKTIGGGDD	acetylation	[1]
60	FSETGAGKHVPRAVF	ubiquitination	[2]
96	PEQLITGKEDAANNY	ubiquitination	[2]
163	RLSVDYGKKSKLEFS	ubiquitination	[2]
304	EPANQMVKCDPRHGK	ubiquitination	[2]
326	YRGDVVPKDVNAAIA	ubiquitination	[2]
336	NAAIATIKTKRSIQF	ubiquitination	[2]
338	AIATIKTKRSIQFVD	ubiquitination	[2]
370	VPGGDLAKVQRAVCM	ubiquitination	[2]
394	AWARLDHKFDLMYAK	ubiquitination	[2]
401	KFDLMYAKRAFVHWY	ubiquitination	[2]

Reference

 [1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. 

Sequence Annotation

 NP_BIND 142 148 GTP (Potential).
 MOD_RES 40 40 N6-acetyllysine (By similarity).
 MOD_RES 41 41 Phosphothreonine (By similarity).
 MOD_RES 48 48 Phosphoserine (By similarity).
 MOD_RES 232 232 Phosphoserine (By similarity).
 MOD_RES 334 334 Phosphothreonine (By similarity).
 MOD_RES 339 339 Omega-N-methylarginine (By similarity).
 MOD_RES 340 340 Phosphoserine (By similarity).
 MOD_RES 432 432 Phosphotyrosine (By similarity).
 MOD_RES 439 439 Phosphoserine (By similarity).
 MOD_RES 451 451 Phosphotyrosine (By similarity).
 CROSSLNK 326 326 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 370 370 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; GTP-binding; Isopeptide bond; Methylation; Microtubule; Nucleotide-binding; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 451 AA 

Protein Sequence

Gene Ontology

 GO:0005881; C:cytoplasmic microtubule; IDA:MGI.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0005200; F:structural constituent of cytoskeleton; IDA:MGI.
 GO:0071353; P:cellular response to interleukin-4; IDA:MGI.
 GO:0000226; P:microtubule cytoskeleton organization; IDA:MGI.
 GO:0006457; P:protein folding; TAS:Reactome.
 GO:0051258; P:protein polymerization; IEA:InterPro. 

Interpro

 IPR002452; Alpha_tubulin.
 IPR008280; Tub_FtsZ_C.
 IPR000217; Tubulin.
 IPR018316; Tubulin/FtsZ_2-layer-sand-dom.
 IPR023123; Tubulin_C.
 IPR017975; Tubulin_CS.
 IPR003008; Tubulin_FtsZ_GTPase. 

Pfam

 PF00091; Tubulin
 PF03953; Tubulin_C 

SMART

 SM00864; Tubulin
 SM00865; Tubulin_C 

PROSITE

 PS00227; TUBULIN 

PRINTS

 PR01162; ALPHATUBULIN.
 PR01161; TUBULIN.