CPLM-012455

Genbank Nucleotide ID

Mortality factor 4-like protein 2

Protein Synonyms/Alias

MORF-related gene X protein; Protein MSL3-2; Transcription factor-like protein MRGX

Homo sapiens (Human)

Position	Peptide	Type	References
23	AEEENFKKPTRSNMQ	acetylation	[1]
49	KTAGPQQKNLEPALP	ubiquitination	[2, 3, 4, 5]
116	VESEEAFKNRMEVKV	ubiquitination	[1, 5]
130	VKIPEELKPWLVEDW	ubiquitination	[3]
144	WDLVTRQKQLFQLPA	ubiquitination	[5]
167	EEYANCKKSQGNVDN	ubiquitination	[5]
175	SQGNVDNKEYAVNEV	ubiquitination	[3, 6]
277	LFTASDYKVASAEYH	ubiquitination	[6]

[1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[2] A data set of human endogenous protein ubiquitination sites.
Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266]
[3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[4] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]

Functional Description

Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the MSIN3A complex which acts to repress transcription by deacetylation of nucleosomal histones.

DOMAIN 117 288 MRG.
MOD_RES 71 71 Phosphoserine.
MOD_RES 262 262 Phosphotyrosine (By similarity).
MOD_RES 267 267 Phosphoserine (By similarity).

Chromatin regulator; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; Growth regulation; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005730; C:nucleolus; IDA:UniProtKB.
GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
GO:0051155; P:positive regulation of striated muscle cell differentiation; IEA:Compara.
GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
GO:0001558; P:regulation of cell growth; NAS:UniProtKB.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR008676; MRG.
IPR026541; MRG_dom.