CPLM-019787

Genbank Nucleotide ID

Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha

Protein Synonyms/Alias

PIP5K1-alpha; PtdIns(4)P-5-kinase 1 alpha; 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha; Phosphatidylinositol 4-phosphate 5-kinase type I alpha; PIP5KIalpha

Homo sapiens (Human)

Position	Peptide	Type	References
373	ETDDQFVKKLEHSWK	ubiquitination	[1]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

Catalyzes the phosphorylation of phosphatidylinositol 4- phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs.

DOMAIN 81 449 PIPK.
CROSSLNK 103 103 Glycyl lysine isopeptide (Lys-Gly)

Alternative splicing; ATP-binding; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Isopeptide bond; Kinase; Membrane; Nucleotide-binding; Nucleus; Reference proteome; Transferase; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0030027; C:lamellipodium; IDA:UniProtKB.
GO:0016607; C:nuclear speck; IDA:UniProtKB.
GO:0032587; C:ruffle membrane; IDA:UniProtKB.
GO:0016308; F:1-phosphatidylinositol-4-phosphate 5-kinase activity; IDA:UniProtKB.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0019900; F:kinase binding; IDA:UniProtKB.
GO:0031532; P:actin cytoskeleton reorganization; IMP:UniProtKB.
GO:0032863; P:activation of Rac GTPase activity; IMP:UniProtKB.
GO:0060326; P:cell chemotaxis; IMP:UniProtKB.
GO:0010761; P:fibroblast migration; IEA:Compara.
GO:0048041; P:focal adhesion assembly; IMP:UniProtKB.
GO:0030216; P:keratinocyte differentiation; TAS:UniProtKB.
GO:0006909; P:phagocytosis; TAS:UniProtKB.
GO:0006661; P:phosphatidylinositol biosynthetic process; TAS:Reactome.
GO:0072661; P:protein targeting to plasma membrane; IMP:UniProtKB.
GO:0097178; P:ruffle assembly; IMP:UniProtKB.
GO:0007165; P:signal transduction; TAS:ProtInc.
GO:0044281; P:small molecule metabolic process; TAS:Reactome.

IPR023610; PInositol-4-P-5-kinase.
IPR027483; PInositol-4-P-5-kinase_C.
IPR002498; PInositol-4-P-5-kinase_core.
IPR027484; PInositol-4-P-5-kinase_N.
IPR016034; PInositol-4P-5-kinase_core_sub.