CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024555
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WAS protein family homolog 1 
Protein Synonyms/Alias
 CXYorf1-like protein on chromosome 9; Protein FAM39E 
Gene Name
 WASH1 
Gene Synonyms/Alias
 FAM39E 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
68QVQAIGEKVSLAQAKubiquitination[1, 2]
75KVSLAQAKIEKIKGSubiquitination[1]
138DERALQEKLKDFPVCubiquitination[1]
185TTENLYKKYVFLDPLubiquitination[1]
220DAPLSISKREQLEQQubiquitination[1, 2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
 Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. 
Sequence Annotation
 DOMAIN 361 383 WH2.
 REGION 1 167 WHD1.  
Keyword
 Actin-binding; Complete proteome; Endosome; Membrane; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 465 AA 
Protein Sequence
MTPVRMQHSL AGQTYAVPFI QPDLRREEAV QQMADALQYL QKVSGDIFSR ISQQVEQSRS 60
QVQAIGEKVS LAQAKIEKIK GSKKAIKVFS SAKYPAPGRL QEYGSIFTGA QDPGLQRRPR 120
HRIQSKHRPL DERALQEKLK DFPVCVSTKP EPEDDAEEGL GGLPSNISSV SSLLLFNTTE 180
NLYKKYVFLD PLAGAVTKTH VMLGAETEEK LFDAPLSISK REQLEQQVPE NYFYVPDLGQ 240
VPEIHVPSYL PDLPGIANDL MYSADLGPGI APSAPGTIPE LPTFHTEVAE PLKVDLQDGV 300
LTPPPPPPPP PPAPEVLASA PPLPPSTAAP VGQGARQDDS SSSASPSVQG APREVVDPSG 360
GWATLLESIR QAGGIGKAKL RSMKERKLEK QQQKEQEQVR ATSQGGHLMS DLFNKLVMRR 420
KGISGKGPGA GEGPGGAFVR VSDSIPPLPP PQQPQAEEDE DDWES 465 
Gene Ontology
 GO:0005769; C:early endosome; IDA:UniProtKB.
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0055037; C:recycling endosome; ISS:UniProtKB.
 GO:0055038; C:recycling endosome membrane; IEA:UniProtKB-SubCell.
 GO:0071203; C:WASH complex; IDA:UniProtKB.
 GO:0043014; F:alpha-tubulin binding; IDA:UniProtKB.
 GO:0034314; P:Arp2/3 complex-mediated actin nucleation; ISS:UniProtKB.
 GO:0042147; P:retrograde transport, endosome to Golgi; IMP:UniProtKB. 
Interpro
 IPR021854; WASH_WASD. 
Pfam
 PF11945; WASH_WAHD 
SMART
  
PROSITE
 PS51082; WH2 
PRINTS