CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024262
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Putative ligase MSMEG_5435/MSMEI_5285 
Protein Synonyms/Alias
  
Gene Name
 MSMEG_5435, MSMEI_5285 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
NCBI Taxa ID
 246196 
Lysine Modification
Position
Peptide
Type
References
528LGPGSIPKTPSGKLRpupylation[1]
Reference
 [1] Expansion of the mycobacterial "PUPylome".
 Watrous J, Burns K, Liu WT, Patel A, Hook V, Bafna V, Barry CE 3rd, Bark S, Dorrestein PC.
 Mol Biosyst. 2010 Feb;6(2):376-85. [PMID: 20094657
Functional Description
  
Sequence Annotation
 CROSSLNK 528 528 Isoglutamyl lysine isopeptide (Lys-Gln)  
Keyword
 Complete proteome; Isopeptide bond; Ligase; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 545 AA 
Protein Sequence
MSRFTEKMYR NARTVTTGMV TGEPHEPVRH TWGEVHERAR RIAGGLAAAG IGHGDAVGVL 60
AGFPVEIAPT AQGVWMRGAS LTMLHQPTPR TDLAVWAEDT MNVIGMIELK AVIISEPFLV 120
ATPVLEEKGI LVLKVADLLA ADPIDPVETG EDDLALMQLT SGSTGSPKAV QITHRNIHSN 180
AEAMFVGAKY DVEKDVMVSW LPCFHDMGMV GFLTIPMYFG AELVKVTPMD FLRDTLLWAK 240
LIDKYKGTMT AAPNFAYALL AKRLRRQAKP GDFDLSTLRF ALSGAEPVEP ADVEDFLDAG 300
KPFGLRPEAI LPAYGMAETT LAVSFSPCGE GLVVDEVDAD LLAALRRAVP ATKGNTKRLA 360
SLGPLLNDLE ARVVDENGEV MPARGVGVIE LRGEPVTPGY ITMGGFVPAQ DEYGWYDTGD 420
LGYITEEGNI VVCGRVKDVI IMAGRNIYPT DIERAAGRVE GVRPGCAVAV RLDAGHSRET 480
FAVAVESNAW QDPAEVRRIE HQVAHEVVSE VDVRPRNVVV LGPGSIPKTP SGKLRRANSV 540
SLVTQ 545 
Gene Ontology
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW. 
Interpro
 IPR020845; AMP-binding_CS.
 IPR000873; AMP-dep_Synth/Lig. 
Pfam
 PF00501; AMP-binding 
SMART
  
PROSITE
 PS00455; AMP_BINDING 
PRINTS