UniProt Accession

 ALKB5_HUMAN; Q6P6C2; B4DVJ4; D3DXC6; Q9NXD6 

Genbank Protein ID

 AK000315; AK301107; AC087164; AC109515; CH471196; CH471196; BC062339 

Genbank Nucleotide ID

 BAA91078.1; BAG62706.1; EAW55661.1; EAW55662.1; AAH62339.1 

Protein Name

 RNA demethylase ALKBH5 

Protein Synonyms/Alias

 Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5 

Gene Name

 ALKBH5 

Gene Synonyms/Alias

 ABH5; OFOXD1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
57	PYPVSGAKRKYQEDS	ubiquitination	[1]
102	FSQDECAKIEARIDE	ubiquitination	[2]
116	EVVSRAEKGLYNEHT	ubiquitination	[1, 2]
132	DRAPLRNKYFFGEGY	acetylation	[2, 3, 4]
132	DRAPLRNKYFFGEGY	ubiquitination	[1]
147	TYGAQLQKRGPGQER	ubiquitination	[1, 5, 6]
235	FGCKFQFKPIRVSEP	acetylation	[2, 3, 4, 7]
235	FGCKFQFKPIRVSEP	ubiquitination	[1]
295	DAPRLETKSLSSSVL	ubiquitination	[1]
321	NNRDPALKPKRSHRK	acetylation	[2, 4]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773] 

Functional Description

 Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m(6)A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Requires molecular oxygen, alpha- ketoglutarate and iron. Demethylation of m(6)A mRNA affects mRNA processing and export and is required for spermatogenesis. 

Sequence Annotation

 METAL 204 204 Iron; catalytic (Probable).
 METAL 206 206 Iron; catalytic (By similarity).
 METAL 266 266 Iron; catalytic (Probable).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 64 64 Phosphoserine.
 MOD_RES 69 69 Phosphoserine.
 MOD_RES 71 71 Phosphotyrosine.
 MOD_RES 132 132 N6-acetyllysine.
 MOD_RES 361 361 Phosphoserine.
 MOD_RES 371 371 Phosphoserine (By similarity).
 MOD_RES 374 374 Phosphoserine.
 MOD_RES 384 384 Phosphoserine (By similarity).  

Keyword

 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Differentiation; Dioxygenase; Iron; Metal-binding; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome; Spermatogenesis. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 394 AA 

Protein Sequence

Gene Ontology

 GO:0016607; C:nuclear speck; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0035515; F:oxidative RNA demethylase activity; IDA:UniProtKB.
 GO:0016706; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; IDA:UniProtKB.
 GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0006406; P:mRNA export from nucleus; IMP:UniProtKB.
 GO:0006397; P:mRNA processing; IMP:UniProtKB.
 GO:0035553; P:oxidative single-stranded RNA demethylation; IDA:UniProtKB.
 GO:0001666; P:response to hypoxia; IDA:UniProtKB.
 GO:0007283; P:spermatogenesis; ISS:UniProtKB. 

Interpro

 IPR027450; AlkB-like. 

Pfam

 PF13532; 2OG-FeII_Oxy_2 

SMART

  

PROSITE

  

PRINTS