UniProt Accession

 RPPH_ECOLI; P0A776; Q2MA07; Q46930 

Genbank Protein ID

 U29581; U00096; AP009048 

Genbank Nucleotide ID

 AAB40477.1; AAC75869.1; BAE76899.1 

Protein Name

 RNA pyrophosphohydrolase 

Protein Synonyms/Alias

 (Di)nucleoside polyphosphate hydrolase; Ap5A pyrophosphatase 

Gene Name

 rppH 

Gene Synonyms/Alias

 nudH; ygdP; b2830; JW2798 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
89	RLVRWDTKPVCIGQK	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Master regulator of 5'-dependent mRNA decay. Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage. Preferentially hydrolyzes diadenosine penta-phosphate with ATP as one of the reaction products. Also able to hydrolyze diadenosine hexa- and tetra-phosphate. Has no activity on diadenosine tri-phosphate, ADP-ribose, NADH and UDP- glucose. In the meningitis causing strain E.coli K1, has been shown to play a role in HBMEC (human brain microvascular endothelial cells) invasion in vitro. 

Sequence Annotation

 DOMAIN 6 149 Nudix hydrolase.
 MOTIF 38 59 Nudix box.  

Keyword

 3D-structure; Complete proteome; Hydrolase; Magnesium; Manganese; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 176 AA 

Protein Sequence

Gene Ontology

 GO:0046872; F:metal ion binding; IEA:HAMAP.
 GO:0034353; F:RNA pyrophosphohydrolase activity; IDA:EcoCyc.
 GO:0006402; P:mRNA catabolic process; IMP:EcoCyc.
 GO:0050779; P:RNA destabilization; IMP:EcoliWiki. 

Interpro

 IPR020476; Nudix_hydrolase.
 IPR020084; NUDIX_hydrolase_CS.
 IPR000086; NUDIX_hydrolase_dom.
 IPR015797; NUDIX_hydrolase_dom-like.
 IPR022927; RppH. 

Pfam

 PF00293; NUDIX 

SMART

  

PROSITE

 PS51462; NUDIX
 PS00893; NUDIX_BOX 

PRINTS

 PR00502; NUDIXFAMILY.