CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032065
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RAC-alpha serine/threonine-protein kinase 
Protein Synonyms/Alias
 cDNA FLJ53606, highly similar to RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1) 
Gene Name
 AKT1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
78EMEVSLAKPKHRVTMubiquitination[1]
96EYLKLLGKGTFGKVIubiquitination[1, 2]
206LDYLHSEKNVVYRDLubiquitination[1]
214NVVYRDLKLENLMLDubiquitination[1, 3]
235ITDFGLCKEGIKDGAubiquitination[1]
239GLCKEGIKDGATMKTubiquitination[1]
315RTLGPEAKSLLSGLLubiquitination[1, 4]
338GGGSEDAKEIMQHRFubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 418 AA 
Protein Sequence
MKTERPRPNT FIIRCLQWTT VIERTFHVET PEEREEWTTA IQTVADGLKK QEEEEMDFRS 60
GSPSDNSGAE EMEVSLAKPK HRVTMNEFEY LKLLGKGTFG KVILVKEKAT GRYYAMKILK 120
KEVIVAKDEV AHTLTENRVL QNSRHPFLTA LKYSFQTHDR LCFVMEYANG GELFFHLSRE 180
RVFSEDRARF YGAEIVSALD YLHSEKNVVY RDLKLENLML DKDGHIKITD FGLCKEGIKD 240
GATMKTFCGT PEYLAPEVLE DNDYGRAVDW WGLGVVMYEM MCGRLPFYNQ DHEKLFELIL 300
MEEIRFPRTL GPEAKSLLSG LLKKDPKQRL GGGSEDAKEI MQHRFFAGIV WQHVYEKKLS 360
PPFKPQVTSE TDTRYFDEEF TAQMITITPP DQDDSMECVD SERRPHFPQF SYSASGTA 418 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0030027; C:lamellipodium; IEA:Compara.
 GO:0015630; C:microtubule cytoskeleton; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005819; C:spindle; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0007568; P:aging; IEA:Compara.
 GO:0042640; P:anagen; IEA:Compara.
 GO:0008637; P:apoptotic mitochondrial changes; IEA:Compara.
 GO:0030030; P:cell projection organization; IEA:Compara.
 GO:0071364; P:cellular response to epidermal growth factor stimulus; IEA:Compara.
 GO:0071456; P:cellular response to hypoxia; IEA:Compara.
 GO:0032869; P:cellular response to insulin stimulus; IEA:Compara.
 GO:0007281; P:germ cell development; IEA:Compara.
 GO:0042593; P:glucose homeostasis; IEA:Compara.
 GO:0006006; P:glucose metabolic process; IEA:Compara.
 GO:0015758; P:glucose transport; IEA:Compara.
 GO:0005978; P:glycogen biosynthetic process; IEA:Compara.
 GO:0060709; P:glycogen cell differentiation involved in embryonic placenta development; IEA:Compara.
 GO:0006954; P:inflammatory response; IEA:Compara.
 GO:0060716; P:labyrinthine layer blood vessel development; IEA:Compara.
 GO:0001893; P:maternal placenta development; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0045792; P:negative regulation of cell size; IEA:Compara.
 GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; IEA:Compara.
 GO:0046329; P:negative regulation of JNK cascade; IEA:Compara.
 GO:0006469; P:negative regulation of protein kinase activity; IEA:Compara.
 GO:0090201; P:negative regulation of release of cytochrome c from mitochondria; IEA:Compara.
 GO:0001649; P:osteoblast differentiation; IEA:Compara.
 GO:0018105; P:peptidyl-serine phosphorylation; IEA:Compara.
 GO:0032287; P:peripheral nervous system myelin maintenance; IEA:Compara.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:0030307; P:positive regulation of cell growth; IEA:Compara.
 GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IEA:Compara.
 GO:0010765; P:positive regulation of sodium ion transport; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045907; P:positive regulation of vasoconstriction; IEA:Compara.
 GO:0030163; P:protein catabolic process; IEA:Compara.
 GO:0043491; P:protein kinase B signaling cascade; IEA:Compara.
 GO:0016567; P:protein ubiquitination; IEA:Compara.
 GO:0030334; P:regulation of cell migration; IEA:Compara.
 GO:0010975; P:regulation of neuron projection development; IEA:Compara.
 GO:0032880; P:regulation of protein localization; IEA:Compara.
 GO:0032094; P:response to food; IEA:Compara.
 GO:0051146; P:striated muscle cell differentiation; IEA:Compara.
 GO:0006412; P:translation; IEA:Compara. 
Interpro
 IPR000961; AGC-kinase_C.
 IPR011009; Kinase-like_dom.
 IPR011993; PH_like_dom.
 IPR017892; Pkinase_C.
 IPR001849; Pleckstrin_homology.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase
 PF00433; Pkinase_C 
SMART
 SM00133; S_TK_X
 SM00220; S_TKc 
PROSITE
 PS51285; AGC_KINASE_CTER
 PS50003; PH_DOMAIN
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS