CPLM-005901

Genbank Nucleotide ID

Protein Synonyms/Alias

22 kDa protein; CP-22; CP22; V19

Homo sapiens (Human)

Position	Peptide	Type	References
68	SGIAGGYKPFNLETC	ubiquitination	[1, 2]
127	VDPQELQKALTTMGF	ubiquitination	[1, 2, 3, 4, 5, 6]
146	QAVNSIAKRYSTNGK	ubiquitination	[5]

[1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Calcium-binding protein that modulates excitation- contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels.

DOMAIN 29 64 EF-hand 1.
DOMAIN 70 103 EF-hand 2.
DOMAIN 100 135 EF-hand 3.
DOMAIN 134 169 EF-hand 4.

3D-structure; Alternative splicing; Calcium; Complete proteome; Cytoplasm; Direct protein sequencing; Membrane; Metal-binding; Reference proteome; Repeat; Sarcoplasmic reticulum.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; IDA:BHF-UCL.
GO:0005789; C:endoplasmic reticulum membrane; IDA:BHF-UCL.
GO:0016529; C:sarcoplasmic reticulum; TAS:BHF-UCL.
GO:0033017; C:sarcoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
GO:0030315; C:T-tubule; TAS:BHF-UCL.
GO:0031982; C:vesicle; IEA:Compara.
GO:0030018; C:Z disc; IDA:BHF-UCL.
GO:0005246; F:calcium channel regulator activity; TAS:ProtInc.
GO:0005509; F:calcium ion binding; IDA:BHF-UCL.
GO:0044325; F:ion channel binding; TAS:BHF-UCL.
GO:0005102; F:receptor binding; TAS:ProtInc.
GO:0006816; P:calcium ion transport; IEA:Compara.
GO:0007507; P:heart development; TAS:ProtInc.
GO:0006880; P:intracellular sequestering of iron ion; TAS:ProtInc.
GO:0007517; P:muscle organ development; TAS:ProtInc.
GO:0010459; P:negative regulation of heart rate; IMP:BHF-UCL.
GO:0060315; P:negative regulation of ryanodine-sensitive calcium-release channel activity; IDA:BHF-UCL.
GO:0001508; P:regulation of action potential; TAS:ProtInc.
GO:0086004; P:regulation of cardiac muscle cell contraction; IMP:BHF-UCL.
GO:1901844; P:regulation of cell communication by electrical coupling involved in cardiac conduction; IMP:BHF-UCL.
GO:1901841; P:regulation of high voltage-gated calcium channel activity; IMP:BHF-UCL.
GO:1901077; P:regulation of relaxation of muscle; IMP:BHF-UCL.
GO:0010880; P:regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum; TAS:BHF-UCL.
GO:0007165; P:signal transduction; TAS:UniProtKB.
GO:0006810; P:transport; TAS:ProtInc.

IPR011992; EF-hand-like_dom.
IPR018247; EF_Hand_1_Ca_BS.
IPR002048; EF_hand_dom.

PS00018; EF_HAND_1
PS50222; EF_HAND_2