UniProt Accession

 SPB1_RAT; Q5RJT2 

Genbank Protein ID

 BC086512 

Genbank Nucleotide ID

 AAH86512.1 

Protein Name

 pre-rRNA processing protein FTSJ3 

Protein Synonyms/Alias

 2'-O-ribose RNA methyltransferase SPB1 homolog; Protein ftsJ homolog 3; Putative rRNA methyltransferase 3 

Gene Name

 Ftsj3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
214	DAKFFDPKFAFKEVE	ubiquitination	[1]
570	PPPPTNLKTEKKSSQ	acetylation	[2]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Probable methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation (By similarity). 

Sequence Annotation

 ACT_SITE 157 157 Proton acceptor (By similarity).
 BINDING 56 56 S-adenosyl-L-methionine; via amide
 BINDING 58 58 S-adenosyl-L-methionine; via amide
 BINDING 76 76 S-adenosyl-L-methionine (By similarity).
 BINDING 92 92 S-adenosyl-L-methionine (By similarity).
 BINDING 117 117 S-adenosyl-L-methionine (By similarity).
 MOD_RES 333 333 Phosphoserine (By similarity).
 MOD_RES 335 335 Phosphoserine (By similarity).
 MOD_RES 336 336 Phosphoserine (By similarity).
 MOD_RES 345 345 Phosphoserine (By similarity).
 MOD_RES 353 353 Phosphoserine (By similarity).
 MOD_RES 368 368 Phosphothreonine (By similarity).
 MOD_RES 544 544 Phosphoserine (By similarity).
 MOD_RES 575 575 Phosphoserine (By similarity).
 MOD_RES 659 659 Phosphoserine (By similarity).
 MOD_RES 671 671 Phosphoserine (By similarity).  

Keyword

 Coiled coil; Complete proteome; Methyltransferase; Nucleus; Phosphoprotein; Reference proteome; Ribosome biogenesis; rRNA processing; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 829 AA 

Protein Sequence

Gene Ontology

 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
 GO:0001510; P:RNA methylation; IEA:InterPro.
 GO:0006364; P:rRNA processing; IEA:UniProtKB-KW. 

Interpro

 IPR015507; rRNA-MeTfrase_E.
 IPR012920; rRNA_MeTfrase_Spb1_C.
 IPR024576; rRNA_MeTfrase_Spb1_DUF3381.
 IPR002877; rRNA_MeTrfase_FtsJ_dom. 

Pfam

 PF11861; DUF3381
 PF01728; FtsJ
 PF07780; Spb1_C 

SMART

  

PROSITE

  

PRINTS