CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015155
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein 1 homolog 
Protein Synonyms/Alias
 Zfp-1; Zinc finger protein 475 
Gene Name
 ZFP1 
Gene Synonyms/Alias
 ZNF475 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
74ARQFLILKNQTPIEEubiquitination[1, 2, 3]
88ERGDLFGKALNLNTDubiquitination[1, 2, 3, 4, 5, 6]
105SLRQVPYKYDLYEKTubiquitination[1, 2, 3]
111YKYDLYEKTLKYNSDubiquitination[1, 2, 3, 6]
114DLYEKTLKYNSDLLNubiquitination[1, 2, 3, 6]
129SNRSYAGKQTDECNEubiquitination[2]
139DECNEFGKALLYLKQubiquitination[2]
145GKALLYLKQEKTHSGubiquitination[2]
160VEYSEYNKSGKALSHubiquitination[2]
196CDKAFSFKSLLISHKubiquitination[1, 3]
224CKKTFSHKANLIKHQubiquitination[2]
275YECSECGKTFAQKFEubiquitination[2]
280CGKTFAQKFELTTHQubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
 May be involved in transcriptional regulation. 
Sequence Annotation
 DOMAIN 8 83 KRAB.
 ZN_FING 184 206 C2H2-type 1.
 ZN_FING 212 234 C2H2-type 2.
 ZN_FING 240 262 C2H2-type 3.
 ZN_FING 268 290 C2H2-type 4.
 ZN_FING 296 318 C2H2-type 5.
 ZN_FING 324 346 C2H2-type 6.
 ZN_FING 352 374 C2H2-type 7.
 ZN_FING 380 402 C2H2-type 8.  
Keyword
 Alternative splicing; Complete proteome; DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 407 AA 
Protein Sequence
MNKSQGSVSF TDVTVDFTQE EWEQLDPSQR ILYMDVMLEN YSNLLSVEVW KADDQMERDH 60
RNPDEQARQF LILKNQTPIE ERGDLFGKAL NLNTDFVSLR QVPYKYDLYE KTLKYNSDLL 120
NSNRSYAGKQ TDECNEFGKA LLYLKQEKTH SGVEYSEYNK SGKALSHKAA IFKHQKIKNL 180
VQPFICTYCD KAFSFKSLLI SHKRIHTGEK PYECNVCKKT FSHKANLIKH QRIHTGEKPF 240
ECPECGKAFT HQSNLIVHQR AHMEKKPYEC SECGKTFAQK FELTTHQRIH TGERPYECNE 300
CAKTFFKKSN LIIHQKIHTG EKRYECSECG KSFIQNSQLI IHMRTHTGEK PYECTECGKT 360
FSQRSTLRLH LRIHTGEKPY ECSECGKAFS RKSRLSVHQR VHIGEKP 407 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001909; Krueppel-associated_box.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF01352; KRAB 
SMART
 SM00349; KRAB
 SM00355; ZnF_C2H2 
PROSITE
 PS50805; KRAB
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS