UniProt Accession

 SAHH_YEAST; P39954; D3DLU3 

Genbank Protein ID

 U18796; AY692801; BK006939 

Genbank Nucleotide ID

 AAB64578.1; AAT92820.1; DAA07697.1 

Protein Name

 Adenosylhomocysteinase 

Protein Synonyms/Alias

 AdoHcyase; S-adenosyl-L-homocysteine hydrolase 

Gene Name

 SAH1 

Gene Synonyms/Alias

 YER043C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
21	SLAAFGRKEIELAEH	ubiquitination	[1, 2]
38	PGLMAIRKAYGDVQP	acetylation	[3]
38	PGLMAIRKAYGDVQP	ubiquitination	[2]
47	YGDVQPLKGARIAGC	acetylation	[3]
47	YGDVQPLKGARIAGC	ubiquitination	[2]
127	FAFKDNKKLNLILDD	acetylation	[4]
177	MVKEGKLKVPAINVN	ubiquitination	[2]
189	NVNDSVTKSKFDNLY	ubiquitination	[2]
191	NDSVTKSKFDNLYGC	acetylation	[3]
191	NDSVTKSKFDNLYGC	ubiquitination	[2]
207	ESLVDGIKRATDVML	acetylation	[3]
207	ESLVDGIKRATDVML	ubiquitination	[2]
229	AGYGDVGKGCAAALR	ubiquitination	[2]
319	AWLKANAKECINIKP	ubiquitination	[2]
325	AKECINIKPQVDRYL	ubiquitination	[2]
386	NDKSFREKHIEFQKT	acetylation	[3]
413	ILDEAVAKFHLGNLG	acetylation	[3]
413	ILDEAVAKFHLGNLG	ubiquitination	[1, 2]
428	VRLTKLSKVQSEYLG	acetylation	[3]
428	VRLTKLSKVQSEYLG	ubiquitination	[2]
443	IPEEGPFKADHYRY*	acetylation	[3]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [4] Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures.
 Xie H, Bandhakavi S, Roe MR, Griffin TJ.
 J Proteome Res. 2007 May;6(5):2019-26. [PMID: 17397211] 

Functional Description

 Adenosylhomocysteine is a competitive inhibitor of S- adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine (By similarity). 

Sequence Annotation

 NP_BIND 160 162 NAD (By similarity).
 NP_BIND 223 228 NAD (By similarity).
 NP_BIND 302 304 NAD (By similarity).
 BINDING 58 58 Substrate (By similarity).
 BINDING 134 134 Substrate (By similarity).
 BINDING 159 159 Substrate (By similarity).
 BINDING 189 189 Substrate (By similarity).
 BINDING 193 193 Substrate (By similarity).
 BINDING 194 194 NAD (By similarity).
 BINDING 246 246 NAD (By similarity).
 BINDING 349 349 NAD (By similarity).
 MOD_RES 393 393 Phosphothreonine.  

Keyword

 Complete proteome; Hydrolase; NAD; One-carbon metabolism; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 449 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0004013; F:adenosylhomocysteinase activity; ISS:SGD.
 GO:0006555; P:methionine metabolic process; NAS:SGD.
 GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
 GO:0016259; P:selenocysteine metabolic process; NAS:SGD. 

Interpro

 IPR000043; Adenosylhomocysteinase.
 IPR015878; Ado_hCys_hydrolase_NAD-bd.
 IPR020082; S-Ado-L-homoCys_hydrolase_CS. 

Pfam

 PF05221; AdoHcyase
 PF00670; AdoHcyase_NAD 

SMART

 SM00996; AdoHcyase
 SM00997; AdoHcyase_NAD 

PROSITE

 PS00738; ADOHCYASE_1
 PS00739; ADOHCYASE_2 

PRINTS