CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031372
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Proteasome (Prosome, macropain) subunit, alpha type, 6, isoform CRA_a 
Protein Synonyms/Alias
 Proteasome subunit alpha type-6; cDNA FLJ51729, highly similar to Proteasome subunit alpha type 6 (EC 3.4.25.1) 
Gene Name
 PSMA6 
Gene Synonyms/Alias
 hCG_21177 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
26TSVAVRGKDCAVIVTacetylation[1]
26TSVAVRGKDCAVIVTubiquitination[1, 2, 3, 4, 5]
35CAVIVTQKKVPDKLLubiquitination[5]
36AVIVTQKKVPDKLLDacetylation[1]
36AVIVTQKKVPDKLLDubiquitination[1, 2, 3, 4, 5, 6]
40TQKKVPDKLLDSSTVacetylation[1]
40TQKKVPDKLLDSSTVubiquitination[1, 5, 7]
52STVTHLFKITENIGCubiquitination[5]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Proteasome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 227 AA 
Protein Sequence
MAGLRREYAF KAINQGGLTS VAVRGKDCAV IVTQKKVPDK LLDSSTVTHL FKITENIGCV 60
MTGMTADSRS QVQRARYEAA NWKYKYGYEI PVDMLCKRIA DISQVYTQNA EMRPLGCCMI 120
LIGIDEEQGP QVYKCDPAGY YCGFKATAAG VKQTESTSFL EKKVKKKFDW TFEQTVETAI 180
TCLSTVLSID FKPSEIEVGV VTVENPKFRI LTEAEIDAHL VALAERD 227 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005839; C:proteasome core complex; IEA:InterPro.
 GO:0004298; F:threonine-type endopeptidase activity; IEA:InterPro.
 GO:0051603; P:proteolysis involved in cellular protein catabolic process; IEA:InterPro. 
Interpro
 IPR023332; Proteasome_suA-type.
 IPR001353; Proteasome_sua/b. 
Pfam
 PF00227; Proteasome 
SMART
  
PROSITE
 PS51475; PROTEASOME_A_2 
PRINTS