CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016168
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcriptional regulator Kaiso 
Protein Synonyms/Alias
 Zinc finger and BTB domain-containing protein 33 
Gene Name
 ZBTB33 
Gene Synonyms/Alias
 KAISO; ZNF348 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
5***MESRKLISATDIubiquitination[1]
103DLLDELIKSGQLLGVubiquitination[1, 2, 3]
124GVPLSQVKSISGTAQubiquitination[2]
145LPPDSGDKNLVIQKSubiquitination[1]
151DKNLVIQKSKDEAQDubiquitination[2]
153NLVIQKSKDEAQDNGubiquitination[2]
407NDPGVGSKHLMEGQKubiquitination[3]
414KHLMEGQKIITLDTAubiquitination[1]
449YDIVIPVKDDPDEGEubiquitination[1]
465RLENEIPKTSGSEMAubiquitination[1, 2]
474SGSEMANKRMKVKHDubiquitination[2]
521NIHSWEKKYPCRYCEubiquitination[1]
529YPCRYCEKVFPLAEYubiquitination[1, 4, 5, 6]
570QFMSSHIKSVHSQDPubiquitination[1]
582QDPSGDSKLYRLHPCubiquitination[1, 2, 6]
611SSTIPAMKDDGIGYKubiquitination[1, 2]
623GYKVDTGKEPPVGTTubiquitination[2, 3]
636TTTSTQNKPMTWEDIubiquitination[2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. 
Sequence Annotation
 DOMAIN 32 94 BTB.
 ZN_FING 494 516 C2H2-type 1.
 ZN_FING 522 544 C2H2-type 2.
 ZN_FING 550 573 C2H2-type 3.
 REGION 1 136 Self-association (By similarity).
 REGION 1 103 Interaction with NCOR1.
 REGION 454 672 Interaction with CTNND1 (By similarity).
 REGION 514 638 Required for DNA-binding (By similarity).
 MOTIF 471 480 Nuclear localization signal (By  
Keyword
 3D-structure; Activator; Complete proteome; Cytoplasm; DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Wnt signaling pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 672 AA 
Protein Sequence
MESRKLISAT DIQYSGSLLN SLNEQRGHGL FCDVTVIVED RKFRAHKNIL SASSTYFHQL 60
FSVAGQVVEL SFIRAEIFAE ILNYIYSSKI VRVRSDLLDE LIKSGQLLGV KFIAELGVPL 120
SQVKSISGTA QDGNTEPLPP DSGDKNLVIQ KSKDEAQDNG ATIMPIITES FSLSAEDYEM 180
KKIIVTDSDD DDDDVIFCSE ILPTKETLPS NNTVAQVQSN PGPVAISDVA PSASNNSPPL 240
TNITPTQKLP TPVNQATLSQ TQGSEKLLVS SAPTHLTPNI ILLNQTPLST PPNVSSSLPN 300
HMPSSINLLV QNQQTPNSAI LTGNKANEEE EEEIIDDDDD TISSSPDSAV SNTSLVPQAD 360
TSQNTSFDGS LIQKMQIPTL LQEPLSNSLK ISDIITRNTN DPGVGSKHLM EGQKIITLDT 420
ATEIEGLSTG CKVYANIGED TYDIVIPVKD DPDEGEARLE NEIPKTSGSE MANKRMKVKH 480
DDHYELIVDG RVYYICIVCK RSYVCLTSLR RHFNIHSWEK KYPCRYCEKV FPLAEYRTKH 540
EIHHTGERRY QCLACGKSFI NYQFMSSHIK SVHSQDPSGD SKLYRLHPCR SLQIRQYAYL 600
SDRSSTIPAM KDDGIGYKVD TGKEPPVGTT TSTQNKPMTW EDIFIQQEND SIFKQNVTDG 660
STEFEFIIPE SY 672 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0035556; P:intracellular signal transduction; TAS:ProtInc.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 
Interpro
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF00651; BTB 
SMART
 SM00225; BTB
 SM00355; ZnF_C2H2 
PROSITE
 PS50097; BTB
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS