CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023291
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TBC1 domain family member 2B 
Protein Synonyms/Alias
  
Gene Name
 TBC1D2B 
Gene Synonyms/Alias
 KIAA1055 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43RLCGYLQKLSGKGPLubiquitination[1, 2, 3]
47YLQKLSGKGPLRGYRubiquitination[2]
137WLQELQQKRWEYCNSubiquitination[2]
149CNSLDMVKWDSRTSPubiquitination[2]
163PTPGDFPKGLVARDNubiquitination[2]
184HPNASAEKARNVLAVubiquitination[2]
221SINFYSLKQWGNELKubiquitination[2]
275IVQEEKKKLTPEGNKubiquitination[2]
379VRSSQYDKYFTSSRLubiquitination[2]
621KVRALDLKTLYLTENubiquitination[2]
702LLQKALEKQNPASKQubiquitination[3]
708EKQNPASKQIELDLLubiquitination[2]
809FRDLMSEKLPRLHGHubiquitination[2, 3]
886QDSMSIFKYLRYFTRubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 May act as a GTPase-activating protein (By similarity). 
Sequence Annotation
 DOMAIN 34 139 PH.
 DOMAIN 662 856 Rab-GAP TBC.
 MOD_RES 155 155 Phosphoserine (By similarity).
 MOD_RES 511 511 Phosphoserine (By similarity).
 MOD_RES 957 957 Phosphoserine.  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; GTPase activation; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 963 AA 
Protein Sequence
MPGAGARAEE GGGGGEGAAQ GAAAEPGAGP AREPARLCGY LQKLSGKGPL RGYRSRWFVF 60
DARRCYLYYF KSPQDALPLG HLDIADACFS YQGPDEAAEP GTEPPAHFQV HSAGAVTVLK 120
APNRQLMTYW LQELQQKRWE YCNSLDMVKW DSRTSPTPGD FPKGLVARDN TDLIYPHPNA 180
SAEKARNVLA VETVPGELVG EQAANQPAPG HPNSINFYSL KQWGNELKNS MSSFRPGRGH 240
NDSRRTVFYT NEEWELLDPT PKDLEESIVQ EEKKKLTPEG NKGVTGSGFP FDFGRNPYKG 300
KRPLKDIIGS YKNRHSSGDP SSEGTSGSGS VSIRKPASEM QLQVQSQQEE LEQLKKDLSS 360
QKELVRLLQQ TVRSSQYDKY FTSSRLCEGV PKDTLELLHQ KDDQILGLTS QLERFSLEKE 420
SLQQEVRTLK SKVGELNEQL GMLMETIQAK DEVIIKLSEG EGNGPPPTVA PSSPSVVPVA 480
RDQLELDRLK DNLQGYKTQN KFLNKEILEL SALRRNAERR ERDLMAKYSS LEAKLCQIES 540
KYLILLQEMK TPVCSEDQGP TREVIAQLLE DALQVESQEQ PEQAFVKPHL VSEYDIYGFR 600
TVPEDDEEEK LVAKVRALDL KTLYLTENQE VSTGVKWENY FASTVNREMM CSPELKNLIR 660
AGIPHEHRSK VWKWCVDRHT RKFKDNTEPG HFQTLLQKAL EKQNPASKQI ELDLLRTLPN 720
NKHYSCPTSE GIQKLRNVLL AFSWRNPDIG YCQGLNRLVA VALLYLEQED AFWCLVTIVE 780
VFMPRDYYTK TLLGSQVDQR VFRDLMSEKL PRLHGHFEQY KVDYTLITFN WFLVVFVDSV 840
VSDILFKIWD SFLYEGPKVI FRFALALFKY KEEEILKLQD SMSIFKYLRY FTRTILDARK 900
LISISFGDLN PFPLRQIRNR RAYHLEKVRL ELTELEAIRE DFLRERDTSP DKGELVSDEE 960
EDT 963 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005097; F:Rab GTPase activator activity; IEA:InterPro.
 GO:0032851; P:positive regulation of Rab GTPase activity; IEA:GOC. 
Interpro
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR000195; Rab-GTPase-TBC_dom. 
Pfam
 PF00566; RabGAP-TBC 
SMART
 SM00233; PH
 SM00164; TBC 
PROSITE
 PS50003; PH_DOMAIN
 PS50086; TBC_RABGAP 
PRINTS