CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016680
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc fingers and homeoboxes protein 2 
Protein Synonyms/Alias
 Alpha-fetoprotein regulator 1; AFP regulator 1; Regulator of AFP; Zinc finger and homeodomain protein 2 
Gene Name
 Zhx2 
Gene Synonyms/Alias
 Afr1; Raf 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
542EKSQGQLKTLEDSFLubiquitination[1]
599LDSMGSGKKGSDAVAubiquitination[1]
664EYDQLAAKTGLVRTEubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Acts as a transcriptional repressor. Represses the promoter activity of the CDC25C gene stimulated by NFYA (By similarity). 
Sequence Annotation
 ZN_FING 78 101 C2H2-type 1.
 ZN_FING 110 133 C2H2-type 2.
 DNA_BIND 263 324 Homeobox 1.
 DNA_BIND 439 501 Homeobox 2.
 DNA_BIND 530 591 Homeobox 3.
 DNA_BIND 628 690 Homeobox 4.
 REGION 195 358 Required for homodimerization (By
 REGION 263 497 Required for interaction with NFYA (By
 REGION 263 446 Required for repressor activity (By
 REGION 317 446 Required for nuclear localization (By
 MOD_RES 16 16 Phosphoserine.  
Keyword
 Complete proteome; DNA-binding; Homeobox; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 836 AA 
Protein Sequence
MASKRKSTTP CMVRTSQVLE QDMLEEADRA KDKGAGMPQS DVTKDSWAAE PEHSSKETEV 60
VEVKSMGENL SKKLQGGYEC KYCPYSTQNL NEFTEHVDMQ HPNVILNPLY VCAECNFTTK 120
KYDSLSDHNS KFHPGETNFK LKLIKRNNQT VLEQSIEATN HVVPITASGP GSSDNDPGVS 180
VGKTPMTKTG KLKADAKKVP KKPDEAAPEN HMEGTARLVT DTAEILARLG SVELLQDSLG 240
HVMPSVQLPP NINLVPKVPV PLNTTKYNSA LDTNATMINS FNKFPYPTQA ELSWLTAASK 300
HPEEHIRIWF ATQRLKHGIS WSPEEVEEAR KKMFNGTIQS VPPTITVLPA QLTPTKVSQP 360
ILQTALPCQI LGQPSLVLTQ VTSGSTTVSC SPITLAVAGV TNHGQKRPLV TPQAAPEPKR 420
PHIAQVPEPP PKVANTPLTP ASDRKKTKLQ IAHLKASFLQ SQFPDDAEVY RLIEVTGLAR 480
SEIKKWFSDH RYRCQRGIVH ITSESLAKDQ MAITGTRHGR TYHVYPDFAP QKFKEKSQGQ 540
LKTLEDSFLK SSFPTQAEVE RLRVETKLSR REIDSWFSER RKLRDSMEQA VLDSMGSGKK 600
GSDAVAPNGA LSRLDQLSGA QLAGSLPSPS SAIVQNQEQV HLLRSTFART QWPTPQEYDQ 660
LAAKTGLVRT EIVRWFKENR CLLKTGTLSW LEQYQRHHMS DDRGRDAVSR KVAKQVAESP 720
KNGSEAAHQY AKDPKALSEE DSEKLVPRMK VGGDPTKDCL AGKPSEATSD RSEGSRDGQG 780
SEENEESGIV DFVEVTVGEE DAISEKWGSW SRRVAEGTVE RADSDSDSTP AEAGQA 836 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0046982; F:protein heterodimerization activity; ISS:UniProtKB.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; ISS:UniProtKB.
 GO:0003714; F:transcription corepressor activity; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006402; P:mRNA catabolic process; IDA:MGI.
 GO:0045665; P:negative regulation of neuron differentiation; IDA:MGI.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0035019; P:somatic stem cell maintenance; IDA:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001356; Homeodomain.
 IPR009057; Homeodomain-like.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like. 
Pfam
 PF00046; Homeobox 
SMART
 SM00389; HOX
 SM00355; ZnF_C2H2 
PROSITE
 PS00027; HOMEOBOX_1
 PS50071; HOMEOBOX_2
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS