CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006273
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Elongation factor Tu 
Protein Synonyms/Alias
 EF-Tu; P-40 
Gene Name
 tuf 
Gene Synonyms/Alias
 tufA; BSU01130 
Created Date
 July 27, 2013 
Organism
 Bacillus subtilis (strain 168) 
NCBI Taxa ID
 224308 
Lysine Modification
Position
Peptide
Type
References
38AITTVLHKKSGKGTAacetylation[1]
42VLHKKSGKGTAMAYDacetylation[1]
178VVKGSALKALEGDAEacetylation[1]
210TPERDTEKPFMMPVEacetylation[1]
254IGLQEENKKTTVTGVacetylation[1]
316AEVYVLSKEEGGRHTacetylation[1]
Reference
 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 
Functional Description
 This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. 
Sequence Annotation
 NP_BIND 19 26 GTP (By similarity).
 NP_BIND 82 86 GTP (By similarity).
 NP_BIND 137 140 GTP (By similarity).  
Keyword
 Complete proteome; Cytoplasm; Elongation factor; GTP-binding; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 396 AA 
Protein Sequence
MAKEKFDRSK SHANIGTIGH VDHGKTTLTA AITTVLHKKS GKGTAMAYDQ IDGAPEERER 60
GITISTAHVE YETETRHYAH VDCPGHADYV KNMITGAAQM DGAILVVSAA DGPMPQTREH 120
ILLSKNVGVP YIVVFLNKCD MVDDEELLEL VEMEVRDLLS EYDFPGDDVP VVKGSALKAL 180
EGDAEWEAKI FELMDAVDEY IPTPERDTEK PFMMPVEDVF SITGRGTVAT GRVERGQVKV 240
GDEVEIIGLQ EENKKTTVTG VEMFRKLLDY AEAGDNIGAL LRGVSREEIQ RGQVLAKPGT 300
ITPHSKFKAE VYVLSKEEGG RHTPFFSNYR PQFYFRTTDV TGIIHLPEGV EMVMPGDNTE 360
MNVELISTIA IEEGTRFSIR EGGRTVGSGV VSTITE 396 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005525; F:GTP binding; IEA:HAMAP.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0003746; F:translation elongation factor activity; IEA:HAMAP.
 GO:0006184; P:GTP catabolic process; IEA:GOC. 
Interpro
 IPR000795; EF_GTP-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR009001; Transl_elong_EF1A/Init_IF2_C.
 IPR004161; Transl_elong_EFTu/EF1A_2.
 IPR004541; Transl_elong_EFTu/EF1A_bac/org.
 IPR004160; Transl_elong_EFTu/EF1A_C.
 IPR009000; Transl_elong_init/rib_B-barrel. 
Pfam
 PF00009; GTP_EFTU
 PF03144; GTP_EFTU_D2
 PF03143; GTP_EFTU_D3 
SMART
  
PROSITE
 PS00301; EFACTOR_GTP 
PRINTS
 PR00315; ELONGATNFCT.