UniProt Accession

 HMGB1_MOUSE; P63158; P07155; P27109; P27428 

Genbank Protein ID

 Z11997; X80457; U00431; L38477; BC006586; BC008565; BC083067; BC085090 

Genbank Nucleotide ID

 CAA78042.1; CAA56631.1; AAA20508.1; AAA57042.1; AAH06586.1; AAH08565.1; AAH83067.1; AAH85090.1 

Protein Name

 High mobility group protein B1 

Protein Synonyms/Alias

 High mobility group protein 1; HMG-1 

Gene Name

 Hmgb1 

Gene Synonyms/Alias

 Hmg-1; Hmg1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
3	*****MGKGDPKKPR	acetylation	[1]
7	*MGKGDPKKPRGKMS	acetylation	[1]
8	MGKGDPKKPRGKMSS	acetylation	[1]
12	DPKKPRGKMSSYAFF	acetylation	[1]
28	QTCREEHKKKHPDAS	acetylation	[1]
29	TCREEHKKKHPDASV	acetylation	[1]
30	CREEHKKKHPDASVN	acetylation	[1]
30	CREEHKKKHPDASVN	ubiquitination	[2]
43	VNFSEFSKKCSERWK	ubiquitination	[2]
76	ARYEREMKTYIPPKG	ubiquitination	[2]
82	MKTYIPPKGETKKKF	ubiquitination	[2]
114	SEYRPKIKGEHPGLS	acetylation	[3, 4]
114	SEYRPKIKGEHPGLS	ubiquitination	[2]
127	LSIGDVAKKLGEMWN	acetylation	[1]
127	LSIGDVAKKLGEMWN	ubiquitination	[2]
128	SIGDVAKKLGEMWNN	acetylation	[1, 3, 4]
128	SIGDVAKKLGEMWNN	ubiquitination	[2]
141	NNTAADDKQPYEKKA	acetylation	[4]
146	DDKQPYEKKAAKLKE	acetylation	[3, 4]
146	DDKQPYEKKAAKLKE	succinylation	[4]
146	DDKQPYEKKAAKLKE	ubiquitination	[2]
154	KAAKLKEKYEKDIAA	ubiquitination	[2]
157	KLKEKYEKDIAAYRA	ubiquitination	[2]
172	KGKPDAAKKGVVKAE	acetylation	[1]
173	GKPDAAKKGVVKAEK	acetylation	[1]
177	AAKKGVVKAEKSKKK	acetylation	[1]
180	KGVVKAEKSKKKKEE	acetylation	[1]
182	VVKAEKSKKKKEEED	acetylation	[1]
183	VKAEKSKKKKEEEDD	acetylation	[1]
184	KAEKSKKKKEEEDDE	acetylation	[1]
185	AEKSKKKKEEEDDEE	acetylation	[1]

Reference

 [1] Monocytic cells hyperacetylate chromatin protein HMGB1 to redirect it towards secretion.
 Bonaldi T, Talamo F, Scaffidi P, Ferrera D, Porto A, Bachi A, Rubartelli A, Agresti A, Bianchi ME.
 EMBO J. 2003 Oct 15;22(20):5551-60. [PMID: 14532127]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. 

Sequence Annotation

 DNA_BIND 9 79 HMG box 1.
 DNA_BIND 95 163 HMG box 2.
 MOD_RES 30 30 N6-acetyllysine (By similarity).
 MOD_RES 35 35 Phosphoserine (By similarity).
 MOD_RES 100 100 Phosphoserine (By similarity).
 CROSSLNK 112 112 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Chromosome; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Heparin-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 215 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; IEA:Compara.
 GO:0000793; C:condensed chromosome; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005576; C:extracellular region; TAS:BHF-UCL.
 GO:0017053; C:transcriptional repressor complex; IEA:Compara.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008201; F:heparin binding; IEA:UniProtKB-KW.
 GO:0001786; F:phosphatidylserine binding; TAS:BHF-UCL.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0006288; P:base-excision repair, DNA ligation; IEA:Compara.
 GO:0006954; P:inflammatory response; TAS:BHF-UCL.
 GO:0002437; P:inflammatory response to antigenic stimulus; IEA:Compara.
 GO:2000426; P:negative regulation of apoptotic cell clearance; IDA:BHF-UCL.
 GO:0017055; P:negative regulation of RNA polymerase II transcriptional preinitiation complex assembly; IEA:Compara.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:0043280; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; IEA:Compara.
 GO:0043388; P:positive regulation of DNA binding; IEA:Compara.
 GO:0033151; P:V(D)J recombination; IEA:Compara. 

Interpro

 IPR009071; HMG_box_dom.
 IPR017967; HMG_boxA_CS. 

Pfam

 PF09011; DUF1898
 PF00505; HMG_box 

SMART

 SM00398; HMG 

PROSITE

 PS00353; HMG_BOX_1
 PS50118; HMG_BOX_2 

PRINTS