| Tag | Content |
|---|
| CPLM ID | CPLM-006087 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Pyruvate carboxylase 2 |
Protein Synonyms/Alias | Pyruvic carboxylase 2; PCB 2 |
Gene Name | PYC2 |
Gene Synonyms/Alias | YBR218C; YBR1507 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 370 | ITTEDPSKNFQPDTG | ubiquitination | [1] | | 543 | WRQVLLEKGPSEFAK | ubiquitination | [1] | | 1125 | HKGSLVKKGESIAVL | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. |
Sequence Annotation | DOMAIN 19 471 Biotin carboxylation.
DOMAIN 141 338 ATP-grasp.
DOMAIN 558 825 Carboxyltransferase.
DOMAIN 1102 1169 Biotinyl-binding.
REGION 566 570 Substrate binding (By similarity).
ACT_SITE 313 313 By similarity.
METAL 567 567 Divalent metal cation (By similarity).
METAL 735 735 Divalent metal cation; via carbamate
METAL 765 765 Divalent metal cation (By similarity).
METAL 767 767 Divalent metal cation (By similarity).
BINDING 137 137 ATP (By similarity).
BINDING 221 221 ATP (By similarity).
BINDING 256 256 ATP (By similarity).
BINDING 639 639 Substrate (By similarity).
BINDING 899 899 Substrate (By similarity).
MOD_RES 735 735 N6-carboxylysine (By similarity).
MOD_RES 1136 1136 N6-biotinyllysine (By similarity). |
Keyword | ATP-binding; Biotin; Complete proteome; Cytoplasm; Gluconeogenesis; Ligase; Metal-binding; Multifunctional enzyme; Nucleotide-binding; Reference proteome; Zinc. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1180 AA |
Protein Sequence | MSSSKKLAGL RDNFSLLGEK NKILVANRGE IPIRIFRSAH ELSMRTIAIY SHEDRLSMHR 60
LKADEAYVIG EEGQYTPVGA YLAMDEIIEI AKKHKVDFIH PGYGFLSENS EFADKVVKAG 120
ITWIGPPAEV IDSVGDKVSA RHLAARANVP TVPGTPGPIE TVQEALDFVN EYGYPVIIKA 180
AFGGGGRGMR VVREGDDVAD AFQRATSEAR TAFGNGTCFV ERFLDKPKHI EVQLLADNHG 240
NVVHLFERDC SVQRRHQKVV EVAPAKTLPR EVRDAILTDA VKLAKVCGYR NAGTAEFLVD 300
NQNRHYFIEI NPRIQVEHTI TEEITGIDIV SAQIQIAAGA TLTQLGLLQD KITTRGFSIQ 360
CRITTEDPSK NFQPDTGRLE VYRSAGGNGV RLDGGNAYAG ATISPHYDSM LVKCSCSGST 420
YEIVRRKMIR ALIEFRIRGV KTNIPFLLTL LTNPVFIEGT YWTTFIDDTP QLFQMVSSQN 480
RAQKLLHYLA DLAVNGSSIK GQIGLPKLKS NPSVPHLHDA QGNVINVTKS APPSGWRQVL 540
LEKGPSEFAK QVRQFNGTLL MDTTWRDAHQ SLLATRVRTH DLATIAPTTA HALAGAFALE 600
CWGGATFDVA MRFLHEDPWE RLRKLRSLVP NIPFQMLLRG ANGVAYSSLP DNAIDHFVKQ 660
AKDNGVDIFR VFDALNDLEQ LKVGVNAVKK AGGVVEATVC YSGDMLQPGK KYNLDYYLEV 720
VEKIVQMGTH ILGIKDMAGT MKPAAAKLLI GSLRTRYPDL PIHVHSHDSA GTAVASMTAC 780
ALAGADVVDV AINSMSGLTS QPSINALLAS LEGNIDTGIN VEHVRELDAY WAEMRLLYSC 840
FEADLKGPDP EVYQHEIPGG QLTNLLFQAQ QLGLGEQWAE TKRAYREANY LLGDIVKVTP 900
TSKVVGDLAQ FMVSNKLTSD DIRRLANSLD FPDSVMDFFE GLIGQPYGGF PEPLRSDVLR 960
NKRRKLTCRP GLELEPFDLE KIREDLQNRF GDIDECDVAS YNMYPRVYED FQKIRETYGD 1020
LSVLPTKNFL APAEPDEEIE VTIEQGKTLI IKLQAVGDLN KKTGQREVYF ELNGELRKIR 1080
VADKSQNIQS VAKPKADVHD THQIGAPMAG VIIEVKVHKG SLVKKGESIA VLSAMKMEMV 1140
VSSPADGQVK DVFIKDGESV DASDLLVVLE EETLPPSQKK 1180 |
Gene Ontology | |
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Pfam | |
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PROSITE | |
PRINTS | |