CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012346
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein 460 
Protein Synonyms/Alias
 Zinc finger protein 272; Zinc finger protein HZF8 
Gene Name
 ZNF460 
Gene Synonyms/Alias
 ZNF272 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
117GTDPQSEKLHGKMSLubiquitination[1]
194EQILPRVKPYDCPECubiquitination[1]
203YDCPECGKAFGKSKHubiquitination[1]
231YKCLECGKDFNRRSHubiquitination[1]
250QRTHNGDKPFVCSECubiquitination[1]
278QRVHSGEKPFVCNECubiquitination[1]
287FVCNECGKAFTYRSNubiquitination[1]
300SNFVLHNKSHNEKKPubiquitination[1, 2]
305HNKSHNEKKPFACSEubiquitination[1]
306NKSHNEKKPFACSECubiquitination[1]
337HTGERPFKCLECGKAubiquitination[3, 4]
343FKCLECGKAFNCRSHubiquitination[1, 5]
362ERIHTGEKPFVCSQCubiquitination[1]
390ERAHTGEKPFECKECubiquitination[1]
395GEKPFECKECGKAFSubiquitination[1]
418FNIHTGEKPYECLQCubiquitination[1, 5]
427YECLQCGKAFTRMSGubiquitination[1]
446QWIHTGEKPYVCIQCubiquitination[1]
474FSIHTGEKPYECVECubiquitination[1, 5]
483YECVECGKAFNRRSPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
 May be involved in transcriptional regulation. 
Sequence Annotation
 DOMAIN 13 99 KRAB.
 ZN_FING 196 218 C2H2-type 1.
 ZN_FING 224 246 C2H2-type 2.
 ZN_FING 252 274 C2H2-type 3.
 ZN_FING 280 302 C2H2-type 4.
 ZN_FING 308 330 C2H2-type 5.
 ZN_FING 336 358 C2H2-type 6.
 ZN_FING 364 386 C2H2-type 7.
 ZN_FING 392 414 C2H2-type 8.
 ZN_FING 420 442 C2H2-type 9.
 ZN_FING 448 470 C2H2-type 10.
 ZN_FING 476 498 C2H2-type 11.  
Keyword
 Complete proteome; DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 562 AA 
Protein Sequence
MAAAWMAPAQ ESVTFEDVAV TFTQEEWGQL DVTQRALYVE VMLETCGLLV ALGDSTKPET 60
VEPIPSHLAL PEEVSLQEQL AQGVPRYSYL GQAMDQDGPS EMQEYFLRPG TDPQSEKLHG 120
KMSLEHEGLA TADGICSMMI QNQVSPEDAL YGFDSYGPVT DSLIHEGENS YKFEEMFNEN 180
CFLVQHEQIL PRVKPYDCPE CGKAFGKSKH LLQHHIIHTG EKPYKCLECG KDFNRRSHLT 240
RHQRTHNGDK PFVCSECGRT FNRGSHLTRH QRVHSGEKPF VCNECGKAFT YRSNFVLHNK 300
SHNEKKPFAC SECGKGFYES TALIQHFIIH TGERPFKCLE CGKAFNCRSH LKQHERIHTG 360
EKPFVCSQCG KAFTHYSTYV LHERAHTGEK PFECKECGKA FSIRKDLIRH FNIHTGEKPY 420
ECLQCGKAFT RMSGLTRHQW IHTGEKPYVC IQCGKAFCRT TNLIRHFSIH TGEKPYECVE 480
CGKAFNRRSP LTRHQRIHTA EKSHEPIQSG NVSCESTDLI QHSIIHTESS PVSAVNMETP 540
SIAAHSSSLD INGFIVEETL PL 562 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001909; Krueppel-associated_box.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF01352; KRAB 
SMART
 SM00349; KRAB
 SM00355; ZnF_C2H2 
PROSITE
 PS50805; KRAB
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS