CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004391
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription factor E2-alpha 
Protein Synonyms/Alias
 Class B basic helix-loop-helix protein 21; bHLHb21; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; Kappa-E2-binding factor; Transcription factor 3; TCF-3; Transcription factor ITF-1 
Gene Name
 TCF3 
Gene Synonyms/Alias
 BHLHB21; E2A; ITF1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
34PLPVTNGKGRPASLAacetylation[1]
334SSGDALGKALASIYSacetylation[1]
Reference
 [1] Mapping acetylation sites in E2A identifies a conserved lysine residue in activation domain 1 that promotes CBP/p300 recruitment and transcriptional activation.
 Hyndman BD, Thompson P, Denis CM, Chitayat S, Bayly R, Smith SP, LeBrun DP.
 Biochim Biophys Acta. 2012 May;1819(5):375-81. [PMID: 22207202] 
Functional Description
 Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue- specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E- box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region. 
Sequence Annotation
 DOMAIN 549 602 bHLH.
 REGION 389 425 Leucine-zipper.
 MOTIF 19 27 9aaTAD.
 MOTIF 170 176 Nuclear localization signal (Potential).
 MOD_RES 134 134 Phosphoserine.
 MOD_RES 139 139 Phosphoserine.
 MOD_RES 359 359 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Chromosomal rearrangement; Complete proteome; Differentiation; DNA-binding; Neurogenesis; Nucleus; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 654 AA 
Protein Sequence
MNQPQRMAPV GTDKELSDLL DFSMMFPLPV TNGKGRPASL AGAQFGGSGL EDRPSSGSWG 60
SGDQSSSSFD PSRTFSEGTH FTESHSSLSS STFLGPGLGG KSGERGAYAS FGRDAGVGGL 120
TQAGFLSGEL ALNSPGPLSP SGMKGTSQYY PSYSGSSRRR AADGSLDTQP KKVRKVPPGL 180
PSSVYPPSSG EDYGRDATAY PSAKTPSSTY PAPFYVADGS LHPSAELWSP PGQAGFGPML 240
GGGSSPLPLP PGSGPVGSSG SSSTFGGLHQ HERMGYQLHG AEVNGGLPSA SSFSSAPGAT 300
YGGVSSHTPP VSGADSLLGS RGTTAGSSGD ALGKALASIY SPDHSSNNFS SSPSTPVGSP 360
QGLAGTSQWP RAGAPGALSP SYDGGLHGLQ SKIEDHLDEA IHVLRSHAVG TAGDMHTLLP 420
GHGALASGFT GPMSLGGRHA GLVGGSHPED GLAGSTSLMH NHAALPSQPG TLPDLSRPPD 480
SYSGLGRAGA TAAASEIKRE EKEDEENTSA ADHSEEEKKE LKAPRARTSP DEDEDDLLPP 540
EQKAEREKER RVANNARERL RVRDINEAFK ELGRMCQLHL NSEKPQTKLL ILHQAVSVIL 600
NLEQQVRERN LNPKAACLKR REEEKVSGVV GDPQMVLSAP HPGLSEAHNP AGHM 654 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0000788; C:nuclear nucleosome; IEA:Compara.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0043234; C:protein complex; IDA:UniProtKB.
 GO:0005667; C:transcription factor complex; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0003677; F:DNA binding; IDA:UniProtKB.
 GO:0070888; F:E-box binding; IDA:UniProtKB.
 GO:0046982; F:protein heterodimerization activity; IDA:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:BHF-UCL.
 GO:0003713; F:transcription coactivator activity; IDA:UniProtKB.
 GO:0070644; F:vitamin D response element binding; IDA:BHF-UCL.
 GO:0002326; P:B cell lineage commitment; IDA:UniProtKB.
 GO:0048468; P:cell development; IEA:Compara.
 GO:0000080; P:G1 phase of mitotic cell cycle; IDA:UniProtKB.
 GO:0043966; P:histone H3 acetylation; IEA:Compara.
 GO:0043967; P:histone H4 acetylation; IEA:Compara.
 GO:0033152; P:immunoglobulin V(D)J recombination; IDA:MGI.
 GO:0042692; P:muscle cell differentiation; TAS:Reactome.
 GO:0001779; P:natural killer cell differentiation; IEA:Compara.
 GO:0048541; P:Peyer's patch development; IEA:Compara.
 GO:0030890; P:positive regulation of B cell proliferation; IMP:UniProtKB.
 GO:0045787; P:positive regulation of cell cycle; IDA:UniProtKB.
 GO:0051149; P:positive regulation of muscle cell differentiation; TAS:Reactome.
 GO:0045666; P:positive regulation of neuron differentiation; ISS:UniProtKB.
 GO:0051091; P:positive regulation of sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:BHF-UCL.
 GO:0050821; P:protein stabilization; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0032496; P:response to lipopolysaccharide; IEA:Compara.
 GO:0033077; P:T cell differentiation in thymus; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IDA:UniProtKB. 
Interpro
 IPR011598; bHLH_dom. 
Pfam
 PF00010; HLH 
SMART
 SM00353; HLH 
PROSITE
 PS50888; BHLH 
PRINTS