CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001175
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Citrate synthase, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 CS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
34SASSTNLKDILADLIubiquitination[1]
43ILADLIPKEQARIKTubiquitination[1, 2, 3, 4]
76YGGMRGMKGLVYETSacetylation[5]
76YGGMRGMKGLVYETSubiquitination[1, 6]
103FSIPECQKLLPKAKGubiquitination[2, 3, 4, 6]
321QLQKEVGKDVSDEKLacetylation[7]
321QLQKEVGKDVSDEKLubiquitination[8]
327GKDVSDEKLRDYIWNacetylation[9]
327GKDVSDEKLRDYIWNubiquitination[3, 4]
366CQREFALKHLPNDPMacetylation[9]
366CQREFALKHLPNDPMubiquitination[1, 6]
375LPNDPMFKLVAQLYKacetylation[9]
382KLVAQLYKIVPNVLLacetylation[9]
382KLVAQLYKIVPNVLLubiquitination[1, 2, 3, 4, 6, 10]
393NVLLEQGKAKNPWPNacetylation[9]
393NVLLEQGKAKNPWPNubiquitination[1]
395LLEQGKAKNPWPNVDubiquitination[6]
450GFPLERPKSMSTEGLubiquitination[1]
459MSTEGLMKFVDSKSGubiquitination[2, 3, 4, 10]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
 [8] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [9] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [10] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
  
Sequence Annotation
 ACT_SITE 301 301 By similarity.
 ACT_SITE 347 347 By similarity.
 ACT_SITE 402 402 By similarity.
 MOD_RES 76 76 N6-acetyllysine; alternate (By
 MOD_RES 76 76 N6-succinyllysine; alternate (By
 MOD_RES 327 327 N6-acetyllysine.
 MOD_RES 375 375 N6-acetyllysine.
 MOD_RES 382 382 N6-acetyllysine.
 MOD_RES 393 393 N6-acetyllysine.
 MOD_RES 395 395 N6,N6,N6-trimethyllysine (By similarity).  
Keyword
 Acetylation; Complete proteome; Direct protein sequencing; Methylation; Mitochondrion; Reference proteome; Transferase; Transit peptide; Tricarboxylic acid cycle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 466 AA 
Protein Sequence
MALLTAAARL LGTKNASCLV LAARHASASS TNLKDILADL IPKEQARIKT FRQQHGKTVV 60
GQITVDMMYG GMRGMKGLVY ETSVLDPDEG IRFRGFSIPE CQKLLPKAKG GEEPLPEGLF 120
WLLVTGHIPT EEQVSWLSKE WAKRAALPSH VVTMLDNFPT NLHPMSQLSA AVTALNSESN 180
FARAYAQGIS RTKYWELIYE DSMDLIAKLP CVAAKIYRNL YREGSGIGAI DSNLDWSHNF 240
TNMLGYTDHQ FTELTRLYLT IHSDHEGGNV SAHTSHLVGS ALSDPYLSFA AAMNGLAGPL 300
HGLANQEVLV WLTQLQKEVG KDVSDEKLRD YIWNTLNSGR VVPGYGHAVL RKTDPRYTCQ 360
REFALKHLPN DPMFKLVAQL YKIVPNVLLE QGKAKNPWPN VDAHSGVLLQ YYGMTEMNYY 420
TVLFGVSRAL GVLAQLIWSR ALGFPLERPK SMSTEGLMKF VDSKSG 466 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IDA:UniProtKB.
 GO:0004108; F:citrate (Si)-synthase activity; IDA:UniProtKB.
 GO:0005975; P:carbohydrate metabolic process; IDA:UniProtKB.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0006099; P:tricarboxylic acid cycle; TAS:Reactome. 
Interpro
 IPR016142; Citrate_synth-like_lrg_a-sub.
 IPR002020; Citrate_synthase-like.
 IPR016141; Citrate_synthase-like_core.
 IPR019810; Citrate_synthase_AS.
 IPR010109; Citrate_synthase_euk. 
Pfam
 PF00285; Citrate_synt 
SMART
  
PROSITE
 PS00480; CITRATE_SYNTHASE 
PRINTS
 PR00143; CITRTSNTHASE.